Insecticidal crystal proteins of Bacillus thuringiensis.
Herman Höfte,H R Whiteley +1 more
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TLDR
In this article, a classification for crystal protein genes of Bacillus thuringiensis is presented, based on the insecticidal spectra and the amino acid sequences of the encoded proteins.About:
This article is published in Microbiological Research.The article was published on 1989-06-01 and is currently open access. It has received 2044 citations till now. The article focuses on the topics: Delta endotoxin & Bacillus thuringiensis.read more
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The effect of oxygen on the sporulation, δ-endotoxin synthesis and toxicity of Bacillus thuringiensis H14
TL;DR: It was found that the optimal conditions for spore yield and for δ-endotoxin yield were not the same, even though sporulation andδ- endotoxin formation proceed simultaneously during the fermentation process.
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Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae)
TL;DR: The patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans and exhibited even higher toxic activities against the insect, resulting in more than 90% mortality.
Journal ArticleDOI
Preparation of cyanobacterial peptide toxin as a biopesticide against cotton pests
TL;DR: Glycine-rich peptide toxin of cyanobacterium Scytonema MKU 106 was purified and shows that at high concentrations it will act as an anti-feeding compound for Stylepta derogata (leaf-roller) larvae of the cotton crop and at low concentrations it acts as a biopesticide.
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Deletion of aprA and nprA genes for alkaline protease A and neutral protease A from bacillus thuringiensis: effect on insecticidal crystal proteins.
Yuping Tan,William P Donovan +1 more
TL;DR: Enhanced yield of certain crystal proteins can be obtained by deletion of the genes aprA and nprA which are the major extracellular proteases of B. thuringiensis.
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A Comparison of Maize Stalk Rot Occurrence in Bt and Non-Bt Hybrids
TL;DR: Field experiments indicate that, although specific Bt events in some years may cause reductions in stalk rot, the overall effect of Bt transformation on stalk rot occurrence is highly variable.
References
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Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
TL;DR: An algorithm has been developed which identifies alpha-helices involved in the interactions of membrane proteins with lipid bilayers and which distinguishes them from helices in soluble proteins, and suggests four transmembrane helices and a surface-seeking helix in fragment B, the moiety known to have trans Membrane function.
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Transgenic plants protected from insect attack
Mark Vaeck,Arlette Reynaerts,Herman Höfte,Stefan Jansens,Marc De Beuckeleer,Caroline Dean,Caroline Dean,Marc Zabeau,Marc Van Montagu,Jan Leemans +9 more
TL;DR: The Gram-positive bacterium Bacillus thuringiensis produces proteins which are specifically toxic to a variety of insect species, and modified genes have been derived from bt2, a toxin gene cloned from one Bacillus strain.
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Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
TL;DR: Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.
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Insect Tolerant Transgenic Tomato Plants
David A. Fischhoff,Katherine S. Bowdish,Katherine S. Bowdish,Frederick J. Perlak,Pamela G. Marrone,Sheila McCormick,Sheila McCormick,Jeanne G. Niedermeyer,Duff A. Dean,Kuniko Kusano-Kretzmer,Ernest J. Mayer,Dean E. Rochester,Stephen G. Rogers,Robert T. Fraley +13 more
TL;DR: The structure of an insect control protein gene from Bacillus thuringiensis var.
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Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo
Wendy E. Thomas,David J. Ellar +1 more
TL;DR: With the exception of the Lepidopteran cell line, Choristoneura fumiferana 63 CF1, this soluble crystal delta-endotoxin protein showed no in vitro or in vivo toxicity, and no haemolytic activity.