Journal ArticleDOI
Insights Into the Rrf2 Repressor Family - the Structure of Cymr, the Global Cysteine Regulator of Bacillus Subtilis.
William Shepard,William Shepard,Olga Soutourina,Olga Soutourina,Emmanuelle Courtois,Emmanuelle Courtois,Patrick England,Patrick England,Ahmed Haouz,Ahmed Haouz,Isabelle Martin-Verstraete,Isabelle Martin-Verstraete +11 more
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TLDR
The global regulator CymR represses the transcription of a large set of genes involved in cystine uptake and cysteine biosynthesis in Bacillus’subtilis and Staphylococcus’aureus and hints at a possible location of the Fe–S centre associated with several Rrf2‐type regulators.Abstract:
The global regulator CymR represses the transcription of a large set of genes involved in cystine uptake and cysteine biosynthesis in Bacillus subtilis and Staphylococcus aureus. This repressor belongs to the widespread and poorly characterized Rrf2 family of regulators. The crystal structure of CymR from B. subtilis reveals a biologically active dimer, where each monomer folds into two tightly packed domains: a DNA-binding domain, which houses a winged helix-turn-helix (wHTH) motif; and a long dimerization domain, which places the wHTH motifs at the extremes. This architecture explains how these small regulators can span 23-27-bp DNA targets. The wHTH motif of CymR resembles those of the GntR superfamily of regulators, such as FadR and HutC. Superimposing the FadR wHTH motifs bound to their DNA fragments onto the wHTH motifs of the CymR dimer structure suggests that the DNA target and/or the protein must undergo some conformational changes upon binding. The CymR structure also hints at a possible location of the Fe-S centre associated with several Rrf2-type regulators.read more
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Studies of IscR reveal a unique mechanism for metal-dependent regulation of DNA binding specificity
Senapathy Rajagopalan,Sarah J. Teter,Petrus H. Zwart,Richard G. Brennan,Kevin J. Phillips,Kevin J. Phillips,Patricia J. Kiley +6 more
TL;DR: A unique mechanism of ligand-mediated DNA site recognition is suggested, whereby metallocluster ligation relocates a protein-specificity determinant to expand DNA target-site selection, allowing a broader transcriptomic response by holo-IscR.
Journal ArticleDOI
Structural and functional changes in the gut microbiota associated to Clostridium difficile infection
Ana Elena Pérez-Cobas,Alejandro Artacho,Stephan J. Ott,Andrés Moya,María José Gosalbes,Amparo Latorre +5 more
TL;DR: It is found that CDI patients showed, in general, lower diversity and richness than non-infected, as well as an overrepresentation of members of the families Bacteroidaceae, Enterococcaceae, Lactobacillaceae and Clostridium clusters XI and XIVa.
Journal ArticleDOI
Characterization of the [2Fe-2S] cluster of Escherichia coli transcription factor IscR.
Angela S. Fleischhacker,Audria Stubna,Kuang Lung Hsueh,Yisong Guo,Sarah J. Teter,Justin C. Rose,Thomas C. Brunold,John L. Markley,Eckard Münck,Patricia J. Kiley +9 more
TL;DR: Characterization of anaerobically isolated IscR using resonance Raman, Mössbauer, and nuclear magnetic resonance spectroscopies leads to the proposal that the [2Fe-2S] cluster does not have full cysteinyl ligation, and Mutagenesis studies indicate that the highly conserved His107 residue is essential for cluster ligation.
Journal ArticleDOI
Fe-S proteins that regulate gene expression.
TL;DR: The recent findings that have provided invaluable insight into the mechanism and function of these highly significant Fe-S regulatory proteins are reviewed.
Journal ArticleDOI
Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-contaminated soil.
Shixue Zheng,Shixue Zheng,Jing Su,Liang Wang,Rong Yao,Dan Wang,Yujia Deng,Rui Wang,Gejiao Wang,Christopher Rensing +9 more
TL;DR: C. testosteroni S44 could be very useful for bioremediation in heavy metal(loid) polluted soils due to the ability to both reduce toxic Se(VI) and Se(IV) to non-toxic Se (0) under aerobic conditions and to tolerate multiple heavy and transition metals.
References
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The Protein Data Bank
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Journal ArticleDOI
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Journal ArticleDOI
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E. Krissinel,Kim Henrick +1 more
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