Journal ArticleDOI
Interactions in multispecies biofilms: do they actually matter?
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TLDR
The recent focus on complex bacterial communities has led to the recognition of interactions across species boundaries, particularly pronounced in multispecies biofilms, where synergistic interactions impact the bacterial distribution and overall biomass produced.About:
This article is published in Trends in Microbiology.The article was published on 2014-02-01. It has received 400 citations till now.read more
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Implementation of Novel Technologies in HTPD - (Bio-) 3D-Printing and Microfluidics
Abstract: The investigation of complex interactions and the multitude of influencing parameters in biotechnological processes requires a huge number of single experiments. To increase the amount of information gained from the available sample volume, approaches of miniaturizing the investigated unit operations are developed. Also the time invested in experimentation is reduced by parallelized procedures and the manual effort is drastically decreased by the application of robotic instrumentation such as liquid-handling stations (LHS). LHS are widely used tools in the field of bioprocess development. Their ability of automated handling of liquids and miniaturized reaction vessels (e.g. multiwell plates) enables high-throughput screening of a variety of experimental conditions. These miniaturized, parallelized and automated investigations are a central element of high-throughput process development (HTPD) and are well-established in biotechnological research in academia as well as in industry. Only recently the established process development has been further improved by new concepts. These include e.g. in silico approaches for the automated data handling and the modeling of processes as well as the reorganization of workflows and unit operations. However, for the generation of innovative approaches and their fast integration into process development, novel technologies are to be implemented in HTPD. The scope of this thesis is to broaden the ambit of LHS as toolbox for process development. For this purpose, new technological strategies are to be combined and integrated in automated experimental workflows on LHS. A key element in this work is the application of 3D-printing techniques. 3D-printing is defined as the layerwise built-up of three dimensional objects. Currently, multiple printing techniques are available with a huge amount of different printing materials. The availability of 3D-printer in the entrylevel segment and furthermore the possibility of ordering 3D-printed parts from service providers opens a broad circle of users access to 3D-printing. The simple usability in comparison with conventional fabrication methods allows also laypersons the fast realization of technical concepts. This strategy is most commonly referred to as rapid prototyping. In this thesis, rapid prototyping was used to develop new approaches for the implementation of microfluidics an bioprinting in LHS-based high-throughput experimentation. In the first part, the applicability of the ’microfluidics-on-liquid handling station’ (μF-on-LHS) system for different biotechnological areas was investigated. This system has been developed as a preparatory work for this dissertation. It combines the advantages of experiments
Book ChapterDOI
The Influence of the Microbiota on the Etiology of Colorectal Cancer
TL;DR: The theoretical bodyworks through which certain members of the microbiota are believed to cause CRC are explored, the sensing of microbiota-associated molecular patterns by innate immune receptors known as toll-like-receptors (TLRs) and the various strategies aimed at manipulating the microbiota and targeting the TLRs are explored in the hope of developing new treatment approaches.
Journal ArticleDOI
The contribution of microbial activity to soil–water interactions and soil microstructural stability of a silty loam soil under moisture dynamics
TL;DR: In this article , soil moisture dynamics and microbial activity can affect soil structure by promoting particle reorientation, cementation and spatio-temporal pore space remodeling, which contribute to the formation of stable soil structures and basic physico-chemical soil properties.
Journal ArticleDOI
Polymicrobial Biofilm Organization of Staphylococcus aureus and Pseudomonas aeruginosa in a Chronic Wound Environment
Cassandra Pouget,Catherine Dunyach-Remy,Chloé Magnan,Alix Pantel,Albert Sotto,Jean-Philippe Lavigne +5 more
TL;DR: The impact of the chronic wound environment on biofilm formation and on bacterial lifecycle inside the biofilm was confirmed and planktonic biofilm-detached bacteria showed decreased growth, overexpression of genes encodingBiofilm formation, and an increase in the mature biofilm biomass formed.
Journal ArticleDOI
Microbial Interspecies Interactions and Their Impact on the Emergence and Spread of Antimicrobial Resistance.
TL;DR: This work identifies factors by which interspecies interactions might influence resistance evolution and distinguish between their effect on (a) the emergence of a resistant mutant and (b) the spread of this resistance throughout the population.
References
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Journal ArticleDOI
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Journal ArticleDOI
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