Journal ArticleDOI
Interdigitated comb-like electrodes for continuous separation of malignant cells from blood using dielectrophoresis.
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TLDR
The present technique could potentially be applied to identify incident cancer at a stage and size that is not yet detectable by standard diagnostic techniques (imaging and biochemical testing), Alternatively, it may also be used to detect cancer recurrences.Abstract:
In this paper, a method for continuous flow separation of circulating malignant cells from blood in a microfluidic device using dielectrophoresis is discussed. Separation of MDA231 breast cancer cells after mixing with normal blood cells was achieved with a level of accuracy that enabled precise counting of the malignant cells, separation and eventually, sub-culturing. MDA231 cells were separated from the blood to a daughter channel using two pairs of interdigitated activated comb-like electrode structures. All experiments are performed with conductivity adjusted medium samples. The electrode pairs were positioned divergent and convergent with respect to the flow. The AC signals used in the separation are 20 V peak-to-peak with frequencies of 10-50 kHz. The separation is based on balance of magnitude of the dielectrophoretic force and hydrodynamic force. The difference in response between circulating malignant cells and normal cells at a certain band of alternating current frequencies was used for rapid separation of cancer cells from blood. The significance of these experimental results is discussed in this paper, with detailed reporting on the suspension medium, preparation of cells, flow condition and the fabrication process of the microfluidic chip. The present technique could potentially be applied to identify incident cancer at a stage and size that is not yet detectable by standard diagnostic techniques (imaging and biochemical testing). Alternatively, it may also be used to detect cancer recurrences.read more
Citations
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Journal ArticleDOI
Microfluidic approaches for cancer cell detection, characterization, and separation
Jian Chen,Jason Li,Yu Sun +2 more
TL;DR: The working principles and experimental results of key microfluidic platforms for cancer cell detection, characterization, and separation based on cell-affinity micro-chromatography, magnetic activated micro-sorting, and cellular biophysics are summarized.
Journal ArticleDOI
Isolation of circulating tumor cells by dielectrophoresis.
Peter R. C. Gascoyne,Sangjo Shim +1 more
TL;DR: The force equilibrium method of dielectrophoretic field-flow fractionation (DEP-FFF) is shown to offer higher discrimination and throughput than earlier DEP trapping methods and to be applicable to clinical studies.
Journal ArticleDOI
Dielectrophoretic separation of bioparticles in microdevices: a review.
TL;DR: This paper reviews studies particularly related to dielectrophoretic separation in microfluidic devices and a comprehensive review is provided starting from the most simplified effective moment Stokes‐drag (EMSD) method to the most advanced interface resolved method.
Journal ArticleDOI
Technologies for label-free separation of circulating tumor cells: from historical foundations to recent developments
Chao Jin,Sarah M. McFaul,Simon P. Duffy,Xiaoyan Deng,Peyman Tavassoli,Peyman Tavassoli,Peter C. Black,Peter C. Black,Hongshen Ma,Hongshen Ma +9 more
TL;DR: This review reviews biophysical label-free technologies that have been developed for CTC separation, including techniques based on filtration, hydrodynamic chromatography, and dielectrophoresis and discusses requirements for subsequent characterization of CTCs.
References
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An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids
TL;DR: This work presents an alternative paradigm--a fully integrated and reconfigurable droplet-based "digital" microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids, and demonstrates reliable and repeatable high-speed transport of microdroplets.
Journal ArticleDOI
Particle separation by dielectrophoresis
TL;DR: The application of dielectrophoresis to particle discrimination, separation, and fractionation is reviewed, some advantages and disadvantages of currently available approaches are considered, and some caveats are noted.
Journal ArticleDOI
Integrated microfluidic devices
David Erickson,Dongqing Li +1 more
TL;DR: In this paper, the authors provide an in-depth look at the state-of-the-art in integrated microfludic devices for a broad range of application areas from on-chip DNA analysis, immunoassays and cytometry to advances in integrated detection technologies for and miniaturized fuel processing devices.
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