Isolation and characterization of revertant cell lines. VI. Susceptibility of revertants to retransformation by simian virus 40 and murine sarcoma virus.
Arthur Vogel,Robert H. Pollack +1 more
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The susceptibility of two classes of revertants of Simian virus 40 (SV40)-transformed 3T3 cells to retransformation by SV40 or murine sarcoma virus (MSV) was studied.Abstract:
The susceptibility of two classes of revertants of Simian virus 40 (SV40)-transformed 3T3 cells to retransformation by SV40 or murine sarcoma virus (MSV) was studied. Both serum-sensitive and density-sensitive revertants are not retransformable by SV40. MSV can transform both types of revertants. The MSV-transformed revertants grow to high cell densities and form colonies when suspended in semi-solid methylcellulose medium, but are unable to grow in 1% calf serum. The MSV-transformed revertants produce infectious MSV and murine leukemia virus and possess the same number of chromosomes as the untransformed revertants.read more
Citations
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Book ChapterDOI
Cell Transformation by RNA Tumor Viruses
TL;DR: The establishment of an assay system for Rous sarcoma virus (RSV) in tissue culture cells (Temin and Rubin, 1958) eventually led to an era of quantitative studies on the mechanism of cellular alteration by viruses.
Book ChapterDOI
The transformation of cell growth and transmogrification of DNA synthesis by simian virus 40.
TL;DR: The hypothesis is that transformation by SV40 unlike PyV is largely explicable in terms of the ability of the 90K T-antigen to initiate DNA synthesis, which can propel the cell into rounds of replication beyond those exhibited by non-transformed cells in the same conditions.
Journal ArticleDOI
Revertants of v-fos-transformed fibroblasts have mutations in cellular genes essential for transformation by other oncogenes
TL;DR: The results suggest that the class I revertants sustained mutations in one or more cellular genes essential for transformation by some, but not all, oncogenes, and suggest the existence of common biochemical pathways for transformation.
Journal ArticleDOI
Transforming genes and gene products of polyoma and SV40.
TL;DR: The small DNA-containing viruses, SV40 and polyoma, transform cells in vitro and induce tumors in vivo and the genetic and biochemical studies defining the sequences important for transformation will be reviewed.
References
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Journal ArticleDOI
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