Journal ArticleDOI
Isolation of an arginine-rich protein from particles of adenovirus type 2.
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TLDR
Polyacrylamide gel electrophoresis showed that particles of adenovirus type 2, disrupted with hot sodium dodecyl sulphate (SDS) and dithiothreitol, contained nine protein components with molecular weights ranging from 190,000 to 11,000.About:
This article is published in Virology.The article was published on 1970-07-01. It has received 92 citations till now. The article focuses on the topics: Polyacrylamide gel electrophoresis & Hexon protein.read more
Citations
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Journal ArticleDOI
The polypeptides of adenovirus: I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12☆
TL;DR: Comparison of nontumorigenic type 2 with tumorigenic types 7A and 12 by double-isotope labeling revealed a generally similar peptide pattern for all types, however, there were distinct differences between the corresponding peptides of all three types.
Journal ArticleDOI
Processing of adenovirus 2-induced proteins
TL;DR: It is proposed that at least two of the adenovirus 2 virion components are derived by the cleavage of higher molecular weight precursor polypeptides, which imply precursor-product relationships for these components.
Journal ArticleDOI
Structural proteins of adenoviruses: II. Purification and characterization of the adenovirus type 2 fiber antigen☆
Ulf Pettersson,Stefan Höglund +1 more
TL;DR: Treatment of penton antigen with pyridine releases free vertex capsomers which are immunologically and morphologically intact and have the ability to induce cytopathic changes in KB-cell cultures.
Book ChapterDOI
The Replication of Herpesviruses
Bernard Roizman,Deirdre Furlong +1 more
TL;DR: This chapter deals with the structure and replication of herpesviruses in susceptible cells and its scope is stated.
Journal ArticleDOI
Genetic analysis of adenovirus type 2 III. Temperature sensitivity of processing viral proteins.
TL;DR: It is proposed that the ts 1 gene codes for or regulates an endoprotease which is responsible for the completion of the last step in virus maturation, that is, the conversion of "young virions" into mature infectious virions by a series of maturation cleavages.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal ArticleDOI
Chromatography of Amino Acids on Sulfonated Polystyrene Resins. An Improved System
TL;DR: A systematic study has been made of the separations of amino acids by elution analysis on columns of synthetic ion exchange resins, demonstrating that synthetic resins are capable of separating most of the common amino acids from one another.
Journal ArticleDOI
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
TL;DR: Electrophoresis in 2.2-2.6% gels gives a fractionation similar to density-gradient centrifugation, and the resolution is greater and more detailed than by centrifugations, and many samples can be analysed simultaneously and rapidly.
Related Papers (5)
The polypeptides of adenovirus: I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12☆
The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion
Structural proteins of adenoviruses: II. Purification and characterization of the adenovirus type 2 fiber antigen☆
Ulf Pettersson,Stefan Höglund +1 more