Journal ArticleDOI
Isolation of renal brush-border membrane vesicles by a low-speed centrifugation; effect of sex hormones on Na+-H+ exchange in rat and mouse kidney.
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TLDR
Na+-H+ exchange and Na+-dependent D-glucose transport in the mouse kidney are under control of androgen hormones, which could be in close connection with the wellknown renotropic action of androgens in themouse.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1986-11-06. It has received 28 citations till now. The article focuses on the topics: Na+/K+-ATPase.read more
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Androgen regulation of rat renal angiotensinogen messenger RNA expression.
TL;DR: Northern and slot blot analyses demonstrated that castration lowered ang-n mRNA levels in the male kidney by greater than or equal to 60% compared with control, suggesting that androgen may be involved with renal ang- n gene regulation.
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Expression of Na+-D-glucose cotransporter SGLT2 in rodents is kidney-specific and exhibits sex and species differences.
Ivan Sabolić,Ivana Vrhovac,Daniela Balen Eror,Maria Gerasimova,Michael Rose,Davorka Breljak,Marija Ljubojević,Hrvoje Brzica,Anne Sebastiani,Serge C. Thal,Christoph Sauvant,Helmut Kipp,Volker Vallon,Hermann Koepsell +13 more
TL;DR: It is indicated that in rodents the expression of SGLT2 is kidney-specific and point to distinct sex and species differences in S GLT2 protein expression that cannot be explained by differences in mRNA.
Journal ArticleDOI
Testosterone replacement therapy and cardiovascular risk.
TL;DR: Until the results of the TRAVERSE trial are available, clinicians should individualize testosterone treatment after having an informed discussion with their patients about the risks and benefits of testosterone replacement therapy.
Journal ArticleDOI
Androgens augment proximal tubule transport
Albert Quan,Sumana Chakravarty,Jian Kang Chen,Jianchun Chen,Samer Loleh,Neel Saini,Raymond C. Harris,Jorge H. Capdevila,Raymond Quigley +8 more
TL;DR: The results suggest that androgens may directly upregulate the proximal tubule renin-angiotensin system, increase the volume reabsorptive rate, and thereby increase extracellular volume and blood pressure and secondarily decrease serum angiotens in II levels.
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Mutational analysis of transmembrane histidines in the amiloride-sensitive Na+/H+ exchanger
TL;DR: Results indicate that H349 affects the interaction of NHE1 with its inhibitors, even though substitutions at this site, per se, do not appear to explain the differences in amiloride sensitivity between different NHE isoforms.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.
TL;DR: Experiments with tritiated NADH are described which demonstrate that this "external" pathway of NADH oxidation resembles stereochemically the NADH-cytochrome c reductase system of liver microsomes, and differs from the respiratory chain-linked NADH dehydrogenase.
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A rapid method for the preparation of microvilli from rabbit kidney
Andrew G. Booth,A. John Kenny +1 more
TL;DR: Marker enzymes for other subcellular components showed the preparation to be essentially free of mitochondria and to be less contaminated with endoplasmic reticulum and baso-lateral plasma membranes than are conventional brush-border preparations.
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Intracellular pH and activation of sea urchin eggs after fertilisation.
James D. Johnson,David Epel +1 more
TL;DR: The intracellular pH of the sea urchin embryo increases 0.3 pH units between 1 and 4 min after fertilisation, which is required for initiating development.
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A high yield preparation for rat kidney brush border membranes Different behaviour of lysosomal markers
TL;DR: Rat kidney cortex slices were homogenized with a polytron in a isoosmotic medium containing 5 mmol/l EGTA and by SDS-polyacrylamide gel electrophoresis, no differences in the protein patterns were observed.