Large-scale identification of leaf senescence-associated genes
Shimon Gepstein,Gazalah Sabehi,Marie-Jeanne Carp,Taleb Hajouj,Mizied Falah Orna Nesher,Inbal Yariv,Chen Dor,Michal Bassani +7 more
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TLDR
The large repertoire of SAGs identified here provides global insights about regulatory, biochemical and cellular events occurring during leaf senescence, and is likely to participate in macromolecule degradation, detoxification of oxidative metabolites, induction of defense mechanisms, and signaling and regulatory events.Abstract:
Leaf senescence is a form of programmed cell death, and is believed to involve preferential expression of a specific set of "senescence-associated genes" (SAGs). To decipher the molecular mechanisms and the predicted complex network of regulatory pathways involved in the senescence program, we have carried out a large-scale gene identification study in a reference plant, Arabidopsis thaliana. Using suppression subtractive hybridization, we isolated approximately 800 cDNA clones representing SAGs expressed in senescing leaves. Differential expression was confirmed by Northern blot analysis for 130 non-redundant genes. Over 70 of the identified genes have not previously been shown to participate in the senescence process. SAG-encoded proteins are likely to participate in macromolecule degradation, detoxification of oxidative metabolites, induction of defense mechanisms, and signaling and regulatory events. Temporal expression profiles of selected genes displayed several distinct patterns, from expression at a very early stage, to the terminal phase of the senescence syndrome. Expression of some of the novel SAGs, in response to age, leaf detachment, darkness, and ethylene and cytokinin treatment was compared. The large repertoire of SAGs identified here provides global insights about regulatory, biochemical and cellular events occurring during leaf senescence.read more
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Phosphorus Dynamics: From Soil to Plant
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TL;DR: In this article, an analysis of changes in global gene expression patterns during developmental leaf senescence in Arabidopsis has identified more than 800 genes that show a reproducible increase in transcript abundance.
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References
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Journal ArticleDOI
Inhibition of Leaf Senescence by Autoregulated Production of Cytokinin
Susheng Gan,Richard M. Amasino +1 more
TL;DR: This result demonstrates that endogenously produced cytokinin can regulate senescence and provides a system to specifically manipulate the senescences program.
Journal ArticleDOI
The molecular biology of leaf senescence
TL;DR: Experiments with transgenic plants and mutants are already shedding light on the role played by cytokinins and ethylene in regulating senescence in leaves, and analysis of the regulatory mechanisms controlling the expression ofSenescence-induced genes will allow the signalling pathways that are involved in the regulation of senescences to be elucidated.
Journal ArticleDOI
Making sense of senescence : Molecular genetic regulation and manipulation of leaf senescence
Susheng Gan,Richard M. Amasino +1 more
TL;DR: This Update summary of physiological and biochemical studies that have contributed to the present understanding of leaf senescence is summarized, current molecular investigations into the regulatory mechanism(s) underlying leafsenescence are discussed, and some molecular approaches toward the manipulation of senescences are reviewed.
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