Journal ArticleDOI
LHRH-like system in the brain of Xenopus laevis daud
TLDR
In this article, the authors used antiserum to synthetic LHRH-secreting neurons and their axonal pathways in the brain of Xenopus laevis to identify three groups of immunoreactive neurons: the first is a paired group of cells scattered near the two telencephalic ventricles; the second group lies near the preoptic recess; the third group occurs in the ventral wall of the infundibulum.Abstract:
Rabbit antiserum to synthetic LHRH was used with the immunofluorescence technique to identify the LHRH-secreting neurons and their axonal pathways in the brain of Xenopus laevis. Three groups of immunoreactive neurons were identified: the first, in the telencephalon, is a paired group of cells scattered near the two telencephalic ventricles; the second group lies near the preoptic recess; the third group occurs in the ventral wall of the infundibulum. Two principal neuronal pathways were observed: Fibres originating from the dorsally located telencephalic neurons converge on the cephalic median plane where they form a single bundle behind the telencephalic furrow. This bundle descends towards the anterior border of the preoptic recess where it divides into two nerve bundles which pass on either side of the preoptic recess, run above the optic chiasma then cross the infundibular floor and finally terminate in the median eminence. The second pathway is more direct. The more ventrally located telencephalic LHRH cells give rise to this second pathway. Their axons converge with the other LHRH fibres near the lateral border of the preoptic recess. Most of the LHRH nerve fibres terminate in the median eminence although some terminate near the paired pars tuberalis. No reaction was observed after the use of antiserum absorbed with synthetic antigen.read more
Citations
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Journal ArticleDOI
Hypothalamic control of the pars distalis in fishes, amphibians, and reptiles.
Journal ArticleDOI
Localization and identification of Neuropeptide Y (NPY)-like immunoreactivity in the frog brain
J. M. Danger,J. Guy,M. Benyamina,Sylvie Jégou,François Leboulenger,Jean Côté,Marie-Christine Tonon,Georges Pelletier,Hubert Vaudry +8 more
TL;DR: The distribution of neuropeptide Y (NPY) in the central nervous system of the frog Rana ridibunda was determined by immunofluorescence using a highly specific antiserum and revealed that infundibulum, preoptic area and telencephalon extracts contained a major peptide bearing NPY-like immunoreactivity.
Book ChapterDOI
Immunohistochemistry of luteinizing hormone-releasing hormone-producing neurons of the vertebrates.
TL;DR: Observations of various LH- RH-reactive axonal tract endings exhibit that besides its major, direct, prehypophysotrophic action through the hypophyseoportal system, LH-RH may also be distributed by the blood of the vascular organ of the lamina terminalis, as neuroendocrine integrator neurons.
Journal ArticleDOI
Testicular regulation in nonmammalian vertebrates.
TL;DR: Attempts to trace the antecedents of the vertebrate pituitary to this specialized structure in tunicates or the wheel organ and Hatschek’s pit of Amphioxus have been singularly unsuccessful.
Journal ArticleDOI
Multiple forms of gonadotropin-releasing hormone in amphibian brains.
TL;DR: Several forms of gonadotropin-releasing hormone (GnRH)-like molecules were found in brains of both anurans and urodeles, and it is likely that two separate genes in amphibians code for the distinct primary structures of the molecules.
References
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Journal ArticleDOI
LRF producing cells of the mammalian hypothalamus. A fluorescent antibody study.
TL;DR: The demonstration of perikarya of mediocellular neurones producing LRF, using indirect immunofluorescence on slides and anti synthetic LRF antibodies, requires both their activation and the inhibition of their axoplasmic transport, suggesting that LRF is present in an immunoreactive form, but usually in very low concentrations.
Journal ArticleDOI
Localization of gonadotropin-releasing hormone (Gn-RH) in the hypothalamus of the mouse by immunoperoxidase technique.
TL;DR: The results suggest that Gn-RH may be synthesized in arcuate neurons, and that tanycytes may provide a major pathway for the secretion of this releasing hormone in male and female mice.
Journal ArticleDOI
LH-RH-containing neural elements in the rat hypothalamus.
TL;DR: With the use of rabbit anti-LH-RH serum, a system of delicate nerve fibers and terminals which contain immunoreactive LH-RH can be demonstrated in the median eminence of the rat, corresponding exactly to the tubero-infundibular tract with the aid of classical neurophistological methods.
Journal ArticleDOI
Immunoreactive LH-RH neurons in the hypothalamus identified by light and fluorescent microscopy
TL;DR: The sites of luteinizing hormone-releasing hormone (LH-RH) neurons and their axonal pathways in the hypothalami of rats and mice were studied by the immuno-globulin-peroxidase bridge technique and the immunofluorescent isothiocyanate technique, using rabbit antiserum to synthetic LH-RH.
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