Journal ArticleDOI
Lipid binding properties of a factor necessary for linoleic acid desaturation
Reads0
Chats0
TLDR
Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase, and albumin does not replace the factor.Abstract:
Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly constituted by phosphatidylcholine. Linoleic acid predominates in the fatty acid composition. By NaCl gradient centrifugation and electrophoresis in gelatinized cellulose acetate, the factor behaves like a lipoprotein. The factor binds linoleic acid and linolyl-CoA that are desaturated to γ-linolenic acid when incubated with washed microsomes. Albumin does not replace the factor.read more
Citations
More filters
Journal ArticleDOI
Recent studies of the enzymic synthesis of ricinoleic Acid by developing castor beans.
Robert A. Moreau,Paul K. Stumpf +1 more
TL;DR: Oleate Delta(12)-hydroxylase activity was measured in extracts of developing castor bean seeds and the enzyme system was not saturated by oleoyl-CoA, even at concentrations as high as 200 micromolar.
Journal ArticleDOI
Prevention of diet‐induced fatty liver in experimental animals by the oral administration of a fatty acid bile acid conjugate (FABAC)
Tuvia Gilat,Tuvia Gilat,Alicia Leikin-Frenkel,Ilana Goldiner,Ilana Goldiner,Christine Juhel,Huguette Lafont,Diana Gobbi,Diana Gobbi,Fred M. Konikoff,Fred M. Konikoff +10 more
TL;DR: Oral FABAC therapy prevents/reduces the development of fatty liver in animals consuming a HFD and gains weight similarly to the controls.
Book ChapterDOI
2 Fatty Acid Desaturation
TL;DR: Results suggest that the true substrate was the CoA ester, which is confirmed by analyzing the incubation mixture for radioactive lipid species, and this chapter summarizes the desaturation process in other systems.
Journal ArticleDOI
Modulation of Δ6 and Δ5 rat liver microsomal desaturase activities by dexamethasone-induced factor
TL;DR: The depressing effect of glucocorticoids on Δ6 and Δ5 desaturation capacity depends on an unchanged protein structure present in the cytosolic fraction of the cell and whose biosynthesis is brought about by hormonal induction.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids.
Arthur Kornberg,W.E. Pricer +1 more
Journal ArticleDOI
Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid.
TL;DR: Inhibition and analog studies suggest that the methylene chain of stearyl-CoA assumes an eclipsed ("gauche") conformation at carbon atoms 9,10 in the enzyme-substrate complex of desaturase, and isotope rate effects obtained with deuterated st earyl- CoA derivatives indicate that hydrogen removal is the rate-limiting step of desaturation.