Journal ArticleDOI
Lipids in the Classification and Identification of Coryneform Bacteria Containing Peptidoglycans Based on 2, 4-diaminobutyric Acid
M. D. Collins,Dorothy Jones +1 more
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TLDR
The results of the present study indicate that lipid markers may be of considerable value in the classification and identification of 2, 4-diaminobutyric acid-containing phytopathogenic and saprophytic coryneform bacteria.Abstract:
Strains of 2, 4-diaminobutyric acid-containing coryneform bacteria were degraded by acid methanolysis and the non-hydroxylated fatty acid esters released examined by thin-layer and gas chromatography. The major fatty acid structural types were straight-chain, anteiso- and iso-methyl branched-chain acids. Polar lipids of the test strains were examined by two-dimensional thin-layer chromatography. All strains possessed very characteristic polar lipid patterns consisting of diphosphatidylglycerol, phosphatidylglycerol and a number of uncharacterized glycolipids. Menaquinones (vitamin K) were the sole isoprenoid quinones detected in the test strains. Corynebacterium insidiosum, Cor. michiganense, Cor. nebraskense and Cor. sepedonicum contained unsaturated menaquinones with nine isoprene units, whereas unsaturated menaquinones with 10 isoprene units predominated in strains of Cor. iranicum and Cor. tritici and a strain labelled Arthrobacter sp. The single strain of Cor. aquaticum examined contained comparable amounts of menaquinones with 10 and 11 isoprene units whereas strains of Cor. mediolanum and Flavobacterium dehydrogenans contained major amounts of menaquinones with 11 and 12 isoprene units. The results of the present study indicate that lipid markers may be of considerable value in the classification and identification of 2, 4-diaminobutyric acid-containing phytopathogenic and saprophytic coryneform bacteria.read more
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Book ChapterDOI
4 Lipid and Cell-Wall Analysis in Bacterial Systematics
Kazuo Komagata,Ken-ichiro Suzuki +1 more
TL;DR: Progress in the biochemistry of microorganisms have revealed that cell-component analysis can be effectively applied to bacterial systematics, providing the basis of chemotaxonomy, and DNA homology data contribute to the clarification, not only of the relatedness of two organisms, but also of the evaluation of phenotypic characteristics.
Journal ArticleDOI
Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication.
M D Collins,D Jones +1 more
Journal ArticleDOI
Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance.
TL;DR: Branched-chain fatty acids of the iso and anteiso series occur in many bacteria as the major acyl constituents of membrane lipids and are an important criterion used to aid identification and classification of bacteria.
Journal ArticleDOI
Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa
TL;DR: A numerical study of the fatty acid patterns of 263 reference strains belonging to the genera Arthrobacter, Aureobacterium, Brevibacterium and Cellulomonas found that strains of the species Corynebacterium glutamicum, CoryneBacterium ammoniagenes, and Erysipelothrix rhusiopathiae could be found in distinct clusters, based on quantitative differ...
Journal ArticleDOI
Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia.
Wen-Jun Li,Ping Xu,Peter Schumann,Yu-Qin Zhang,Yu-Qin Zhang,Rüdiger Pukall,Li-Hua Xu,Erko Stackebrandt,Cheng-Lin Jiang +8 more
TL;DR: A Gram-positive, motile, short-rod-shaped strain, isolated from a forest-soil sample collected from Lijiang, Yunnan Province, China, and was investigated using a polyphasic taxonomic approach represents a novel species of the genus Georgenia, for which the nameGeorgenia ruanii sp.
References
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Journal ArticleDOI
Distribution of Menaquinones in Actinomycetes and Corynebacteria
TL;DR: Menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined, and Actinomadura dassonvillei strains had a characteristic pattern of di-, tetra- and hexahydromenaquinONES with 10 isoprene units which was slightly different from the pattern in mixtures of similar quInones from Actinomyces israelii and Act inomyces viscosus.
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