LncRNA MEG3 inhibits the progression of prostate cancer by modulating miR-9-5p/QKI-5 axis.
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TLDR
LncRNA MEG3 was a down‐regulated lncRNA in prostate cancer and impacted the abilities of cell proliferation, migration and invasion, and cell apoptosis rate, this regulation relied on regulating miR‐9‐5p and its targeting gene QKI‐5.Abstract:
This study was designed to detecting the influences of lncRNA MEG3 in prostate cancer. Aberrant lncRNAs expression profiles of prostate cancer were screened by microarray analysis. The qRT-PCR and Western blot were employed to investigating the expression levels of lncRNA MEG3, miR-9-5p and QKI-5. The luciferase reporter assay was utilized to testifying the interactions relationship among these molecules. Applying CCK-8 assay, wound healing assay, transwell assay and flow cytometry in turn, the cell proliferation, migration and invasion abilities as well as apoptosis were measured respectively. LncRNA MEG3 was a down-regulated lncRNA in prostate cancer tissues and cells and could inhibit the expression of miR-9-5p, whereas miR-9-5p down-regulated QKI-5 expression. Overexpressed MEG3 and QKI-5 could decrease the abilities of proliferation, migration and invasion in prostate cancer cells effectively and increased the apoptosis rate. On the contrary, miR-9-5p mimics presented an opposite tendency in prostate cancer cells. Furthermore, MEG3 inhibited tumour growth and up-regulated expression of QKI-5 in vivo. LncRNA MEG3 was a down-regulated lncRNA in prostate cancer and impacted the abilities of cell proliferation, migration and invasion, and cell apoptosis rate, this regulation relied on regulating miR-9-5p and its targeting gene QKI-5.read more
Citations
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Sepideh Mirzaei,Mahshid Deldar Abad Paskeh,Elena Okina,Mohammad Hossein Gholami,Kiavash Hushmandi,M. Hashemi,Azuma Kalu,Ali Zarrabi,Noushin Nabavi,Navid Rabiee,Esmaeel Sharifi,Hassan Karimi-Maleh,Milad Ashrafizadeh,Alan Prem Kumar,Yuzhuo Wang +14 more
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References
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Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression
TL;DR: Findings suggest that lncRNA MEG3, a ceRNA of miR-181 s, could regulate gastric carcinogenesis and may serve as a potential target for antineoplastic therapies.
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Long Non-Coding RNA MEG3 Inhibits Cell Proliferation and Induces Apoptosis in Prostate Cancer.
Gang Luo,Miao Wang,Xinchao Wu,Dan Tao,Xinyuan Xiao,Liang Wang,Fan Min,Fuqing Zeng,Guosong Jiang +8 more
TL;DR: An important role is presented in the molecular etiology of prostate cancer and the potential application of MEG3 in prostate cancer therapy and the underlying mechanism is implicated.
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LncRNA PVT1 regulates prostate cancer cell growth by inducing the methylation of miR-146a.
TL;DR: The study suggested a regulatory relationship between lncRNA PVT1 and miR‐146a during the process of the prostate cancer tumorigenesis, which would contribute to the diagnosis, treatment and prognosis of prostate cancer.