Journal ArticleDOI
Localization of Monoamine Oxidase A and B mRNA in the Rat Brain by In Situ Hybridization
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TLDR
In situ hybridization to visualize MAOA and MAOB mRNAs in the rat brain by using specific cDNA and oligonucleotide probes demonstrates that MAOA mRNA synthesis is wide spread in many catecholaminergic and serotonergic cell groups, whereas MAOB RNA synthesis is far more discrete and limited.Abstract:
Monoamine oxidases A and B (MAOA and MAOB) are the major catabolic isoenzymes of catecholamines and serotonin in the mammalian brain. Although the distribution of the monoamine oxidase protein has been mapped by ligand binding and immunohistochemistry, the sites of MAOA and MAOB synthesis have not been precisely determined. In this study, we used in situ hybridization to visualize MAOA and MAOB mRNA in the rat brain by using specific cDNA and oligonucleotide probes. MAOA mRNA was localized in major monoaminergic cell groups, such as the dorsal vagal complex, the C1/A1 groups, the locus ceruleus, the raphe nuclei, the substantia nigra, and the ventral tegmental area. MAOA mRNA was also found in forebrain structures, such as the cortex, the hippocampus, the thalamus, and the hypothalamus. In contrast to the distribution of MAOA mRNA, high levels of MAOB mRNA were present in only three brain regions: the area postrema, the subfornical organ, and the dorsal raphe. The in situ visualization of MAO mRNA demonstrates that MAOA mRNA synthesis is wide spread in many catecholaminergic and serotonergic cell groups, whereas MAOB mRNA synthesis is far more discrete and limited. The different expression patterns of MAOA and MAOB suggests that may also have different physiological functions.read more
Citations
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Journal ArticleDOI
Monoamine oxidase: from genes to behavior.
Jean C. Shih,K. Chen,M. J. Ridd +2 more
TL;DR: MAO A and B knock-out mice are valuable models for investigating the role of monoamines in psychoses and neurodegenerative and stress-related disorders and show increased reactivity to stress.
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Non–cell autonomous toxicity in neurodegenerative disorders: ALS and beyond
TL;DR: The evidence is reviewed here the evidence that it is the convergence of damage developed within multiple cell types, including within neighboring nonneuronal supporting cells, which is crucial to neuronal dysfunction.
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Diverse actions of ovarian steroids in the serotonin neural system.
TL;DR: The ability of estrogens and progestins to alter the function of the serotonin neural system at various levels provides a cellular mechanism whereby ovarian hormones can impact cognition, mood or arousal, hormone secretion, pain, and other neural circuits.
Journal ArticleDOI
Contributions of non-human primates to neuroscience research.
TL;DR: The role of non-human primates in development of new treatments before phase I clinical trials in patients; basic research on disease pathogenesis; and understanding neurobehavioural outcomes resulting from genotype-environment interactions are discussed.
Journal ArticleDOI
Monoamine oxidase expression during development and aging.
TL;DR: A knowledge of MAO developmental changes not only provides a basis for the investigation of factors regulating MAO expression, but can also contribute to a better understanding of the possible trophic and/or morphogenetic role of monoaminergic neurotransmitters in the developing brain.
References
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The Rat Brain in Stereotaxic Coordinates
George Paxinos,Charles Watson +1 more
TL;DR: This paper presents a meta-analyses of the determinants of earthquake-triggered landsliding in the Czech Republic over a period of 18 months in order to establish a probabilistic framework for estimating the intensity of the earthquake.
Journal ArticleDOI
Aggressive Behavior and Altered Amounts of Brain Serotonin and Norepinephrine in Mice Lacking MAOA
Olivier Cases,Isabelle Seif,Joseph Grimsby,Patricia Gaspar,Kevin Chen,Sandrine Pournin,Ulrike Müller,Michel Aguet,Charles Babinet,Jean C. Shih,Edward De Maeyer +10 more
TL;DR: Pup behavioral alterations, including trembling, difficulty in righting, and fearfulness were reversed by the serotonin synthesis inhibitor parachlorophenylalanine, and adults manifested a distinct behavioral syndrome, including enhanced aggression in males.
Journal ArticleDOI
cDNA cloning of human liver monoamine oxidase A and B: molecular basis of differences in enzymatic properties.
Bach Alfred,Nancy C. Lan,Deborah L. Johnson,Creed W. Abell,Michael E. Bembenek,Sau-Wah Kwan,Peter H. Seeburg,Jean C. Shih +7 more
TL;DR: Using oligonucleotide probes derived from three sequenced peptide fragments, isolated cDNA clones that encode the A and B forms of monoamine oxidase are isolated and the nucleotide sequences of these cDNAs are determined.
Journal ArticleDOI
Distinct monoamine oxidase A and B populations in primate brain.
TL;DR: These data illustrate the physiological independence of MAO A and B and show that neurons may be specialized for their degradative as well as their synthetic functions.
Journal ArticleDOI
Immunocytochemical demonstration of monoamine oxidase B in brain astrocytes and serotonergic neurons
TL;DR: The specific distribution of MAO-B in the adult central nervous system indicates that the role of MAB in monoamine metabolism may be more specifically defined than previously believed.