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Journal ArticleDOI

Lysis of nitrofurantoin-resistant strain of Vibrio el tor.

Haldar S, +4 more
- 01 Jan 1983 - 
- Vol. 28, Iss: 1, pp 22-27
TLDR
Differences in periplasmic proteins released from Tris-EDTA treated cells of nitrofurantoin-resistant and -sensitive strains were revealed by gel electrophoresis.
Abstract: 
Both nitrofurantoin-sensitive and nitrofurantoin-resistant strains ofVibrio el tor were found to lyze in the presence of Tris-EDTA at alkaline pH. The rate of lysis was appreciably enhanced by lysozyme. The amounts of intracellular components,viz. proteins and carbohydrates, released from the nitrofurantoin-sensitive strain by Tris-EDTA treatment, were significantly lower than those from the nitrofurantoin-resistant strain. Differences in periplasmic proteins released from Tris-EDTA treated cells of nitrofurantoin-resistant and -sensitive strains were revealed by gel electrophoresis.

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References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

Oliver H. Lowry, +3 more
- 01 Nov 1951 - 
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

Colorimetric Method for Determination of Sugars and Related Substances

Michel. DuBois, +4 more
- 01 Jan 1956 - 
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI

Disc electrophoresis – ii method and application to human serum proteins*

Baruch J. Davis
- 16 Dec 2006 - 
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI

The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.

Harold C. Neu, +1 more
- 01 Sep 1965 - 
TL;DR: A new method for releasing most of the inducible alkaline phosphatases and of the cyclic phosphodiesterase in high yield without greatly impairing the viability of the cells is developed.
Journal ArticleDOI

Interaction of Divalent Cations and Polymyxin B With Lipopolysaccharide

Melvin Schindler, +1 more
- 02 Oct 1979 - 
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