Journal ArticleDOI
Magnetic nanoparticles coated with polyaniline to stabilize immobilized trypsin
Jackeline da Costa Maciel,A. A. D. Mercês,Mariana P. Cabrera,W. T. Shigeyosi,S. D. de Souza,M. Olzon-Dionysio,José Domingos Fabris,C. A. Cardoso,David Fernando de Morais Neri,M. P. C. Silva,Luiz B. Carvalho +10 more
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TLDR
In this paper, the synthesis of magnetic nanoparticles via the chemical co-precipitation of Fe 3+ ions and their preparation by coating them with polyaniline was reported.Abstract:
It is reported the synthesis of magnetic nanoparticles via the chemical co-precipitation of Fe 3+ ions and their preparation by coating them with polyaniline. The electronic micrograph analysis showed that the mean diameter for the nanoparticles is ∼15 nm. FTIR, powder X-ray diffraction and Mossbauer spectroscopy were used to understand the chemical, crystallographic and 57Fe hyperfine structures for the two samples. The nanoparticles, which exhibited magnetic behavior with relatively high spontaneous magnetization at room temperature, were identified as being mainly formed by maghemite (γFe2O3). The coated magnetic nanoparticles (sample labeled “mPANI”) presented a real ability to bind biological molecules such as trypsin, forming the magnetic enzyme derivative (sample “mPANIG-Trypsin”). The amount of protein and specific activity of the immobilized trypsin were found to be 13±5 μg of protein/mg of mPANI (49.3 % of immobilized protein) and 24.1±0.7 U/mg of immobilized protein, respectively. After 48 days of storage at 4 ∘C, the activity of the immobilized trypsin was found to be 89 % of its initial activity. This simple, fast and low-cost procedure was revealed to be a promising way to prepare mPANI nanoparticles if technological applications addressed to covalently link biomolecules are envisaged. This route yields chemically stable derivatives, which can be easily recovered from the reaction mixture with a magnetic field and recyclable reused.read more
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Improvement of the stability and activity of immobilized trypsin on modified Fe3O4 magnetic nanoparticles for hydrolysis of bovine serum albumin and its application in the bovine milk.
TL;DR: Immobilized trypsin (TR) was more stable than the free one and demonstrated higher enzymatic activity at elevated temperatures (45-55°C) and in the alkaline pH region (6-10.5) while Fe3O4 NPs-GA-TR retained about 64% of its initial activity during the same storage period.
Journal ArticleDOI
Polyaniline-coated magnetic diatomite nanoparticles as a matrix for immobilizing enzymes
Mariana P. Cabrera,Taciano França da Fonseca,Raquel Varela Barreto de Souza,Caio Rodrigo Dias de Assis,Justiniano Quispe Marcatoma,Jackeline da Costa Maciel,David Fernando de Morais Neri,Fernando Soria,Luiz Bezerra de Carvalho +8 more
TL;DR: In this paper, a composite made from magnetic diatomaceous earth (mDE) coated with polyaniline was used as a matrix for immobilizing industrial enzymes: invertase, β-galactosidase, and trypsin.
Journal ArticleDOI
Effect of synthesis and activation methods on the catalytic properties of silica nanospring (NS)-supported iron catalyst for Fischer-Tropsch synthesis
TL;DR: A nanostructured iron (Fe) catalyst for Fischer-Tropsch synthesis (FTS) was prepared and evaluated using a silica nanospring (NS) support as mentioned in this paper.
Journal ArticleDOI
Enhancement of the catalytic performance of silica nanosprings (NS)-supported iron catalyst with copper, molybdenum, cobalt and ruthenium promoters for Fischer-Tropsch synthesis
TL;DR: In this article, the Fischer-Tropsch synthesis of Fe/Nospring (Fe/NS) catalysts with different metal promoters (Co, Mo, Cu, and Ru) was investigated to investigate effects of these metals on the physico-chemical properties and catalytic behavior of the Fe/NS catalyst.
Journal ArticleDOI
Hydrolysis of tannins by tannase immobilized onto magnetic diatomaceous earth nanoparticles coated with polyaniline.
Juliana Silva de Lima,Mariana P. Cabrera,Cristina Maria de Souza Motta,Attilio Converti,Luiz B. Carvalho +4 more
TL;DR: Even though the tannins content of boldo tea was reduced by only 23–25% after 120 min treatment with either free or immobilized enzyme, mDE-PANI-TANNASE has the advantage of being simply recovered and recycled from the reaction mixture by an outer magnet, thus preventing contamination of the treated tea.
References
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Journal Article
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TL;DR: Biocompatibility, Pharmaceutical and Biomedical Applications L. Harivardhan Reddy,‡ Jose ́ L. Arias, Julien Nicolas,† and Patrick Couvreur*,†.
Journal ArticleDOI
Conductive polymers: towards a smart biomaterial for tissue engineering.
TL;DR: Focusing mainly on polypyrrole, polyaniline and poly(3,4-ethylenedioxythiophene), this work reviews conductive polymers from the perspective of tissue engineering.
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