Journal ArticleDOI
Mapping regulatory genes as candidates for cold and drought stress tolerance in barley
Alessandro Tondelli,Enrico Francia,Delfina Barabaschi,Alessio Aprile,Jeffrey S. Skinner,Eric J. Stockinger,Antonio Michele Stanca,Nicola Pecchioni +7 more
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TLDR
Observations suggest that CBF gene(s) themselves, rather than their two regulators, are at present the best candidates for cold tolerance.Abstract:
Cereal crop yield is greatly affected in many growing areas by abiotic stresses, mainly low temperature and drought. In order to find candidates for the tolerance genes for these stresses, 13 genes encoding for transcription factors and upstream regulators were screened by amplification and SSCP on six parental genotypes of three barley mapping populations ('Nure' x 'Tremois', 'Proctor' x 'Nudinka', and 'Steptoe' x 'Morex'), and mapped as newly developed STS, SNP, and SSCP markers. A new consensus function map was then drawn using the three maps above, including 16 regulatory candidate genes (CGs). The positions of barley cold and drought tolerance quantitative trait loci (QTLs) presently described in the literature were added to the consensus map to find positional candidates from among the mapped genes. A cluster of six HvCBF genes co-mapped with the Fr-H2 cold tolerance QTL, while no QTLs for the same trait were positioned on chromosome 7H, where two putative barley regulators of CBF expression, ICE1 and FRY1, found by homology search, were mapped in this work. These observations suggest that CBF gene(s) themselves, rather than their two regulators, are at present the best candidates for cold tolerance. Four out of 12 drought tolerance QTLs of the consensus map are associated with regulatory CGs, on chromosomes 2H, 5H, and 7H, and two QTLs with effector genes, on chromosomes 5H and 6H. The results obtained could be used to guide MAS applications, allowing introduction into an ideal genotype of favourable alleles of tolerance QTLs.read more
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Journal ArticleDOI
Drought tolerance improvement in crop plants: An integrated view from breeding to genomics
Luigi Cattivelli,Fulvia Rizza,Franz-W. Badeck,Elisabetta Mazzucotelli,Anna M. Mastrangelo,Enrico Francia,Caterina Marè,Alessandro Tondelli,A. Michele Stanca +8 more
TL;DR: Breeders are asked to blend together all knowledge on the traits sustaining yield under drought and to accumulate the most effective QTLs and/or transgenes into elite genotypes without detrimental effects on yield potential, which will lead to new cultivars with high yield potential and high yield stability, that will result in superior performance in dry environments.
Journal ArticleDOI
Inducing drought tolerance in plants: Recent advances
TL;DR: Rapid advance in knowledge on genomics and proteomics will certainly be beneficial to fine-tune the molecular breeding and transformation approaches so as to achieve a significant progress in crop improvement in future.
Journal ArticleDOI
Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality.
Sebastian Worch,K. Rajesh,Vokkaliga T. Harshavardhan,Christof Pietsch,Viktor Korzun,Lissy Kuntze,Andreas Börner,Ulrich Wobus,Marion S. Röder,Nese Sreenivasulu +9 more
TL;DR: The high level of haplotype variation among starch biosynthesis/degradation genes in the progenitors of cultivated barley shows that domestication and breeding have greatly eroded their allelic diversity in current elite cultivars.
Journal ArticleDOI
Drought tolerance in wheat.
TL;DR: This review paper has concentrated on the study of water limitation and its effects on morphological, physiological, biochemical, and molecular responses of wheat with the possible losses caused by drought stress.
Journal ArticleDOI
Coping with drought: stress and adaptive responses in potato and perspectives for improvement.
TL;DR: An overview of past research activity on drought tolerance in potato is presented, highlighting recent advances with examples from other crops and suggesting future research directions.
References
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Journal ArticleDOI
Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms
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Journal ArticleDOI
PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms
TL;DR: This review of recent advances in determining the nature and function of genes with roles in freezing tolerance and the mechanisms involved in low temperature gene regulation and signal transduction concludes that cold acclimation includes the expression of certain cold-induced genes that function to stabilize membranes against freeze-induced injury.