Journal ArticleDOI
Metabolic pathway analysis and dynamic macroscopic model development for lovastatin production by Monascus purpureus using metabolic footprinting concept
A. Seenivasan,J. Satya Eswari,P Sankar,Sathyanarayana N. Gummadi,Tapobrata Panda,Venkateswarlu Ch. +5 more
TLDR
Predicted specific substrate utilization and product excretion rates have been correlated well with the experimental observations, which validate the proposed metabolic pathway developed from metabolic footprinting data.About:
This article is published in Biochemical Engineering Journal.The article was published on 2020-02-15. It has received 8 citations till now. The article focuses on the topics: Flux (metabolism) & Metabolic flux analysis.read more
Citations
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Journal ArticleDOI
Perspectives on Functional Red Mold Rice: Functional Ingredients, Production, and Application.
Feng Yanli,Yu Xiang +1 more
TL;DR: The present perspective summarized the bioactive components of FRMR and their functions, and efficient strategies for FRMR production, future challenges ofFRMR application, and possible directions were proposed.
Journal ArticleDOI
Development of One Pot Strategy for Hyper Production and In Vivo Evaluation of Lovastatin.
Muhammad Azeem,Muhammad Arshad,Saqib Mahmood,Shazia Abrar,Ameer Fawad Zahoor,Sadia Javed,Bisma Tariq,Khizar Hayyat +7 more
TL;DR: The results indicate that selected strain along with 2% NaOH treated sugar cane bagasse was best suitable for bulk production of lovastatin by fermentation and fermented Lovastatin effectively lower the cholesterol level of rats.
Journal ArticleDOI
Development of a prototype device for near real-time surface-enhanced Raman scattering monitoring of biological samples.
Elodie Dumont,Charlotte De Bleye,Gilles Rademaker,Laureen Coic,Julie Horne,Pierre-Yves Sacre,Olivier Peulen,Philippe Hubert,Eric Ziemons +8 more
TL;DR: A new methodology, that enables near real-time, non-invasive and non-destructive SERS monitoring of biological samples, is proposed, which combines solid SERS substrates with an original protein filtering device and an on-field detection by means of a handheld Raman analyser.
Journal ArticleDOI
Morphological and structural characterization of chitin as a substrate for the screening, production, and molecular characterization of chitinase by Bacillus velezensis
Digvijay Dahiya,Akhil Pilli,Pratap Raja Reddy Chirra,Vinay Sreeramula,Nitish V. Mogili,S. Ayothiraman +5 more
TL;DR: Interestingly, the addition of complex nitrogen sources has increased the production of chitinase and a 1.95- and 2.14-fold increase in the enzyme activity was observed with peptone and yeast extract, respectively.
References
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Journal ArticleDOI
Modulation of Polyketide Synthase Activity by Accessory Proteins During Lovastatin Biosynthesis
Jonathan Kennedy,Karine Auclair,Steven G. Kendrew,Cheon-Seok Park,John C. Vederas,C. Richard Hutchinson +5 more
TL;DR: Synthesis of the main nonaketide-derived skeleton was found to require the previously known iterative lovastatin nonAKetide synthase (LNKS), plus at least one additional protein that interacts with LNKS and is necessary for the correct processing of the growing polyketide chain and production of dihydromonacolin L.
Journal ArticleDOI
Network rigidity and metabolic engineering in metabolite overproduction.
TL;DR: This work has shown that overproduction of many metabolites requires significant redirection of flux distributions in the primary metabolism, which may not readily occur following product deregulation because metabolic pathways have evolved to exhibit control architectures that resist flux alterations at branch points.
Journal ArticleDOI
High-throughput classification of yeast mutants for functional genomics using metabolic footprinting
Jess Allen,Hazel M. Davey,David Broadhurst,Jim K. Heald,Jeremy John Rowland,Stephen G. Oliver,Douglas B. Kell +6 more
TL;DR: It is shown that metabolic footprinting is an effective method to classify 'unknown' mutants by genetic defect by using appropriate clustering and machine learning techniques, the latter based on genetic programming.
Journal ArticleDOI
Metabolic footprinting and systems biology: the medium is the message
TL;DR: The principles, experimental approaches and scientific outcomes that have been obtained with this useful and convenient strategy to study the inner structure and behaviour of a system are reviewed.
Journal ArticleDOI
Production of the secondary metabolites gamma-aminobutyric acid and monacolin K by Monascus.
TL;DR: Monascus purpureus CCRC 31615, the strain with the highest amount of monacolin K and GABA productivity, was identified from 16 strains using solid fermentation and its GABA productivity was particularly high.