Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006
TLDR
In this paper, the authors attribute Panton-Valentine leucocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) to clonal lineages by molecular typing with special reference to isolates exhibiting spa type t008/multilocus sequence type (MLST) ST8 [widely disseminated in the USA as ‘community-associated MRSA (caMRSA), USA300’].Abstract:
Objectives: The aim of this paper is to attribute Panton-Valentine leucocidin (PVL)-positive methicillinresistant Staphylococcus aureus (MRSA) to clonal lineages by molecular typing with special reference to isolates exhibiting spa type t008/multilocus sequence type (MLST) ST8 [widely disseminated in the USA as ‘community-associated MRSA (caMRSA) USA300’]. Methods :P VL-positive MRSA (n 5 117) were detected among 4815 MRSA sent to the German National Reference Laboratory for typing. These isolates were analysed by PFGE, spa typing, multilocus sequence typing, grouping of SCCmec elements and PCR detection of arcA, msr(A), mph(B) and the � 6 AT repeat unit in the SACOL0058 sequence. Results: Among the 117 isolates, 80 exhibited spa type t044 (corresponding to MLST ST80) and 23 exhibited spa type t008/MLST ST8. Other spa types were sporadically represented. Further characterization of isolates exhibiting t008/ST8 by PCR [arcA, msr(A), mph(B), � 6 AT repeat signature] indicates the arrival of caMRSA ‘USA300’ in Central Europe. Conclusions: caMRSA ST80 still predominate; however, caMRSA ST8 exhibiting the characteristics of the ‘USA300’ clone became the second most frequent. Routine detection of this clone in clinical bacteriology can be easily performed by PCR.read more
Citations
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Journal ArticleDOI
Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic.
Michael Z. David,Robert S. Daum +1 more
TL;DR: This review details the epidemiology of CA-MRSA strains and the clinical spectrum of infectious syndromes associated with them that ranges from a commensal state to severe, overwhelming infection and addresses the therapy of these infections and strategies for their prevention.
Journal ArticleDOI
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology
Sahreena Lakhundi,Kunyan Zhang +1 more
TL;DR: The origin of MRSA is described, with emphasis on the diverse nature of staphylococcal cassette chromosome mec (SCCmec).
Journal ArticleDOI
The evolution of Staphylococcus aureus.
TL;DR: The latest observations about the structure of SCCmec are described, the techniques used to study the molecular epidemiology and evolution of S. aureus as well as some challenges that researchers face in the future are described.
Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its applicatio
Murchan S,Kaufmann M E,Deplano A,Ryck de R,Struelens M,Zinn C E,Fussig,Salmenlinna S,Vuopio-Varkila J,El Solh N,Cuny C,Witte W,Tassios P T,Legakis N,Leeuwen van W,Belkum van A,Vindel A,Laconcha I,Garaizar J,Haeggman S,Olsson-Liljequist B,Ransjo U,Coombes G,Cookson B +23 more
TL;DR: In this article, the authors compared different European pulsed-fieldgel electrophoresis (PFGE) protocols for MRSA and of the various national MRSA clones themselves.
Journal ArticleDOI
Methicillin-Resistant Staphylococcus aureus (MRSA): Burden of disease and control challenges in Europe
Robin Köck,Karsten Becker,Barry Cookson,J.E.W.C. van Gemert-Pijnen,Stéphan Juergen Harbarth,Jan Kluytmans,Martin Mielke,Georg Peters,Robert Skov,Marc Struelens,Marc Struelens,Evelina Tacconelli,A Navarro Torné,Wolfgang Witte,Alexander W. Friedrich +14 more
TL;DR: The current burden of MRSA infections in healthcare and community settings across Europe is described and the main threats caused by recent changes in the epidemiology of MR SA are outlined, aimed at identifying unmet needs of surveillance, prevention and control ofMRSA in Europe.
References
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Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus.
Mark C. Enright,Nicholas P. J. Day,Catrin E. Davies,Sharon J Peacock,Sharon J Peacock,Brian G. Spratt +5 more
TL;DR: A multilocus sequence typing (MLST) scheme has been developed for Staphylococcus aureus and provides an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the Internet.
Journal ArticleDOI
Involvement of Panton-Valentine Leukocidin—Producing Staphylococcus aureus in Primary Skin Infections and Pneumonia
Gerard Lina,Yves Piémont,Florence Godail-Gamot,Michèle Bes,Marie Odile Peter,Valérie Gauduchon,François Vandenesch,Jerome Etienne +7 more
TL;DR: Panton-Valentine leukocidin genes were detected in 93% of strains associated with furunculosis and in 85% of those associated with severe necrotic hemorrhagic pneumonia (all community-acquired), and it appears that PVL is mainly associated with nec rotic lesions involving the skin or mucosa.
Journal ArticleDOI
Community-acquired methicillin-resistant staphylococcus aureus carrying panton-valentine leukocidin genes: worldwide emergence
François Vandenesch,Timothy S. Naimi,Mark C. Enright,Gerard Lina,Graeme R. Nimmo,Helen Heffernan,Nadia Liassine,Michèle Bes,Timothy Greenland,Reverdy Me,Jerome Etienne +10 more
TL;DR: Analysis of CA-MRSA isolates from the United States, France, Switzerland, Australia, New Zealand, and Western Samoa indicated distinct genetic backgrounds associated with each geographic origin, although predominantly restricted to the agr3 background.
Journal ArticleDOI
The changing epidemiology of Staphylococcus aureus
TL;DR: Strains of methicillin-resistant Staphylococcus aureus, which had been largely confined to hospitals and long-term care facilities, are emerging in the community, and the prevalence of these strains in thecommunity seems likely to increase substantially.
Journal ArticleDOI
Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus
Binh An Diep,Binh An Diep,Steven R. Gill,Richard F. Chang,Richard F. Chang,Tiffany HaiVan Phan,Jason H. Chen,Matthew G. Davidson,Felice Lin,Jessica Lin,Heather A. Carleton,Emmanuel F. Mongodin,George F. Sensabaugh,Francoise Perdreau-Remington +13 more
TL;DR: The most striking feature of the USA300 genome is the horizontal acquisition of a novel mobile genetic element that encodes an arginine deiminase pathway and an oligopeptide permease system that could contribute to growth and survival of USA300.