Patent
Methods and compositions for detecting base pair mismatches
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TLDR
In this article, improved compositions and methods for detecting mutations, including single base changes, in nucleic acid sequences using RNase protection assays are presented. But the present method is not suitable for the detection of single base mismatches.Abstract:
The present invention discloses improved compositions and methods for detecting mutations, including single base changes, in nucleic acid sequences using RNase protection assays. The improvements include concomitant, dramatic reductions in the salt and RNase enzyme concentrations in the RNase digestion reaction mixture which result in greater sensitivity in detecting genetic mutations. Another embodiment of the present invention is kits to be used for the detection of single base mismatches in nucleic acid samples.read more
Citations
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Patent
Materials and methods for detection of nucleic acids
David J. Marshall,James R. Prudent,Christopher B. Scherrill,Gideon Shapiro,Jennifer K. Grenier,Craig S. Richmond,Simona Jurczyk,Jerod L. Ptacin +7 more
TL;DR: In this article, a method for detecting target nucleic acids in a sample using non-natural bases is described, which involves contacting the sample with a reporter comprising a label and a nonnatural base complementary to the single-stranded region of the amplification product.
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Nucleic acid detection method using nucleotide probes enabling both specific capture and detection
Marta Blumenfeld,Michel Bouillot +1 more
TL;DR: In this article, a nucleic acid detection method was proposed, in which a probe was hybridized with a predetermined RNA or DNA in a sample, bearing detection elements and first capture elements on a solid medium.
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Solid support assay systems and methods utilizing non-standard bases
Jennifer K. Grenier,David J. Marshall,James R. Prudent,Craig S. Richmond,Eric B. Roesch,Christopher W. Scherrer,Christopher B. Sherrill,Jerod L. Ptacin +7 more
TL;DR: In this article, a capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target, in some instances, the recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target.
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Modifying double stranded dna to enhance separations by matched ion polynucleotide chromatography
TL;DR: Covalently bound non-polar tags are used to increase the retention times of double stranded polynucleotides on Matched Ion Polynucleotide Chromatography (MIPC) columns.
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Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals
Douglas T. Gjerde,Paul D. Taylor +1 more
TL;DR: In this paper, a method for analyzing a sample of double stranded DNA to determine the presence of a mutation therein comprises contacting the sample with a mutation site binding reagent, and chromatographically separating and detecting the product.
References
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Patent
Process for amplifying nucleic acid sequences
TL;DR: In this article, a process for amplifying any desired specific nucleic acid sequence contained in a mixture of nucleic acids or mixture thereof is described, which can be repeated as often as desired.
Journal ArticleDOI
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
TL;DR: In this paper, a simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described, based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at an SP6 promoter.
Journal ArticleDOI
p53: a frequent target for genetic abnormalities in lung cancer
Takashi Takahashi,Marion M. Nau,Itsuo Chiba,Michael J. Birrer,Richard K. Rosenberg,Michelle Vinocour,Mark L. Levitt,Harvey I. Pass,Adi F. Gazdar,John D. Minna +9 more
TL;DR: These findings, coupled with the previous demonstration of 17p allele loss in lung cancer, strongly implicate p53 as an anti-oncogene whose disruption is involved in the pathogenesis of human lung cancer.
Journal ArticleDOI
Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers.
Kenneth W. Kinzler,Mef Nilbert,Bert Vogelstein,Tracy M. Bryan,Daniel B. Levy,Kelly J. Smith,Antoneite C. Preisinger,Stanley R. Hamilton,Phil Hedge,Alex Markham,M. Carlson,Geoff Joslyn,Joanna Groden,Ray White,Yoshoi Miki,Yasuo Miyoshi,Isamu Nishisho,Yusuke Nakamura +17 more
TL;DR: A gene, MCC, which encodes an 829-amino acid protein with a short region of similarity to the G protein-coupled m3 muscarinic acetylcholine receptor is identified and is a candidate for the putative colorectal tumor suppressor gene located at chromosome region 5q21.
Journal ArticleDOI
Characteristics and Prognostic Implications of Myosin Missense Mutations in Familial Hypertrophic Cardiomyopathy
Hugh Watkins,Anthony Rosenzweig,Dar-San Hwang,Tatjana Levi,William J. McKenna,Christine E. Seidman,Jonathan G. Seidman +6 more
TL;DR: It is suggested that the precise definition of the disease-causing mutation can provide important prognostic information about affected members in families with hypertrophic cardiomyopathy.