Open Access
MicroRNAs: Target Recognition and Regulatory Functions
TLDR
In this article, a review outlines the current understanding of miRNA target recognition in animals and discusses the widespread impact of miRNAs on both the expression and evolution of protein-coding genes.Abstract:
MicroRNAs (miRNAs) are endogenous ∼23 nt RNAs that play important gene-regulatory roles in animals and plants by pairing to the mRNAs of protein-coding genes to direct their posttranscriptional repression. This review outlines the current understanding of miRNA target recognition in animals and discusses the widespread impact of miRNAs on both the expression and evolution of protein-coding genes.read more
Citations
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A Circuitous Route to Noncoding RNA
TL;DR: A class of circular RNAs that regulates microRNAs is abundant in mammalian cells, and like protein-coding RNAs, appear to be linear molecules with 5′ and 3′ termini, reflecting the defined start and end points of RNA polymerase on the DNA template.
Iconographies supplémentaires de l'article : Inhibition of house dust mite–induced allergic airways disease by antagonism of microRNA-145 is comparable to glucocorticoid treatment
TL;DR: The importance of understanding the contribution of miRNAs to pathogenesis of human allergic disease and their potential as novelAnti-inflammatory effects of miR-145 antagonism were comparable to steroid treatment.
Impact of MicroRNA Levels, Target-Site Complementarity, and Cooperativity on Competing Endogenous RNA-Regulated Gene Expression
Rémy Denzler,Alexandra C. Title,Markus Stoffel,Sean E. McGeary,Vikram Agarwal,David P. Bartel +5 more
TL;DR: In this paper, the authors show that although miRNA levels define the extent of repression, they have little effect on the magnitude of the ceRNA expression change required to observe derepression, and they provide quantitative insights into the stoichiometric relationship between miRNAs and target abundance, target-site spacing, and affinity requirements for ceRNA-mediated gene regulation.
deepBase v2.0: Identification, Expression, Evolution and Function of Small RNAs, LncRNAs and Circular RNAs from Deep-sequencing Data. doi:10.1093/nar/gkt1248
TL;DR: DeepBase v2.0 as mentioned in this paper provides the most comprehensive collection of ncRNA-derived small RNAs generated from 588 sRNA-Seq datasets, and a pipeline named lncSeeker is developed to identify 176 680 high-confidence lncRNAs from 14 species.
Dissertation
The roles of the microrna 29 family in cartilage homeostasis and osteoarthritis
TL;DR: The miR-29 family was found to be the negative regulator in both human and murine chondrogenesis, and was also found to involve in murine limb development.
References
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Journal ArticleDOI
MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
Journal ArticleDOI
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
TL;DR: Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin- 4 regulates lin- 14 translation via an antisense RNA-RNA interaction.
Journal ArticleDOI
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
TL;DR: In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of the gene set.
Journal ArticleDOI
Most mammalian mRNAs are conserved targets of microRNAs
TL;DR: This work overhauled its tool for finding preferential conservation of sequence motifs and applied it to the analysis of human 3'UTRs, increasing by nearly threefold the detected number of preferentially conserved miRNA target sites.
Journal ArticleDOI
Circular RNAs are a large class of animal RNAs with regulatory potency
Sebastian Memczak,Marvin Jens,Antigoni Elefsinioti,Francesca Torti,Janna Krueger,Agnieszka Rybak,Luisa Maier,Sebastian D. Mackowiak,Lea H. Gregersen,Mathias Munschauer,Alexander Loewer,Ulrike Ziebold,Markus Landthaler,Christine Kocks,Ferdinand le Noble,Nikolaus Rajewsky +15 more
TL;DR: It is found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7.