Microscopy with microlens arrays: high throughput, high resolution and light-field imaging
Antony Orth,Kenneth B. Crozier +1 more
TLDR
Highly parallelized fluorescence scanning microscopy using a refractive microlens array is demonstrated and the ability to extract different perspective views of a pile of microspheres is demonstrated.Abstract:
We demonstrate highly parallelized fluorescence scanning microscopy using a refractive microlens array. Fluorescent beads and rat femur tissue are imaged over a 5.5 mm x 5.5 mm field of view at a pixel throughput of up to 4 megapixels/s and a resolution of 706 nm. We also demonstrate the ability to extract different perspective views of a pile of microspheres.read more
Citations
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Computational illumination for high-speed in vitro Fourier ptychographic microscopy
TL;DR: In this article, the Fourier ptychographic microscopy (FPM) was used for quantitative phase imaging of unstained live samples in vitro, where the authors demonstrate a new computational illumination technique that achieves a large space-bandwidth-time product, achieving 0.8 NA resolution across a 4× FOV with subsecond capture times.
Journal ArticleDOI
Precise and diffraction-limited waveguide-to-free-space focusing gratings.
Karan K. Mehta,Rajeev J. Ram +1 more
TL;DR: The design and characterization of waveguide grating devices that couple visible-wavelength light at λ = 674 nm from single-mode, high index-contrast dielectric waveguides to free-space beams forming micron-scale diffraction-limited spots a designed distance and angle from the grating are presented.
Journal ArticleDOI
Monolithic polymer microlens arrays with high numerical aperture and high packing density.
Hyukjin Jung,Ki-Hun Jeong +1 more
TL;DR: A novel method for monolithic fabrication of high numerical aperture polymer microlens arrays (high-NA MLAs) with high packing density (PD) at wafer level with high photon collection efficiency with signal-to-noise ratio greater than 50:1 is reported.
Journal ArticleDOI
Switchable Pancharatnam-Berry microlens array with nano-imprinted liquid crystal alignment.
TL;DR: A rapid nano-imprinting technique to pattern the liquid crystal alignment of a Pancharatnam-Berry phase microlens array with fast response time and low operation voltage is reported.
Journal ArticleDOI
Optical fiber bundles: Ultra-slim light field imaging probes
Antony Orth,Martin Ploschner,Emma R. Wilson,Ivan S. Maksymov,Ivan S. Maksymov,Brant C. Gibson +5 more
TL;DR: This work uses the mode structure within fiber bundle cores to extract the spatio-angular description of captured light rays—the light field—enabling digital refocusing, stereo visualization, and surface and depth mapping of microscopic scenes at the distal fiber tip.
References
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Journal ArticleDOI
Globally optimal stitching of tiled 3D microscopic image acquisitions
TL;DR: This work developed a method that, based on the Fourier Shift Theorem, computes all possible translations between pairs of 3D images, yielding the best overlap in terms of the cross-correlation measure and subsequently finds the globally optimal configuration of the whole group of3D images.
Journal ArticleDOI
High-throughput fluorescence microscopy for systems biology
Rainer Pepperkok,Jan Ellenberg +1 more
TL;DR: Fluorescence microscopy is making the transition to a quantitative and high-throughput technology to enable these techniques to be applied to functional genomics experiments.
Journal ArticleDOI
Design, fabrication and testing of microlens arrays for sensors and microsystems
TL;DR: In this article, the authors report on their activities in design, fabrication, characterization and system integration of refractive microlens arrays for sensors and microsystems, including neural networks and multiple pupil imaging systems for photolithography.
Journal ArticleDOI
Cellular imaging in drug discovery
TL;DR: This review describes how cellular imaging technologies contribute to the drug discovery process and addresses both high-content and high-throughput needs.
Journal ArticleDOI
Recording and controlling the 4D light field in a microscope using microlens arrays
TL;DR: A prototype system for simulating exotic microscope illumination modalities and correcting for optical aberrations digitally is described and demonstrated, and two applications for it are demonstrated.
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