Modelling a Silent Epidemic: A Review of the In Vitro Models of Latent Tuberculosis.
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TLDR
A summation of the different NRP models and a critical analysis of their respective advantages and disadvantages relating to their physiological relevance are provided.Abstract:
Tuberculosis (TB) is the primary cause of death by a single infectious agent; responsible for around two million deaths in 2016. A major virulence factor of TB is the ability to enter a latent or Non-Replicating Persistent (NRP) state which is presumed untreatable. Approximately 1.7 billion people are latently infected with TB and on reactivation many of these infections are drug resistant. As the current treatment is ineffective and diagnosis remains poor, millions of people have the potential to reactivate into active TB disease. The immune system seeks to control the TB infection by containing the bacteria in a granuloma, where it is exposed to stressful anaerobic and nutrient deprived conditions. It is thought to be these environmental conditions that trigger the NRP state. A number of in vitro models have been developed that mimic conditions within the granuloma to a lesser or greater extent. These different models have all been utilised for the research of different characteristics of NRP Mycobacterium tuberculosis, however their disparity in approach and physiological relevance often results in inconsistencies and a lack of consensus between studies. This review provides a summation of the different NRP models and a critical analysis of their respective advantages and disadvantages relating to their physiological relevance.read more
Citations
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References
More filters
Journal ArticleDOI
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.
TL;DR: A screening window coefficient, called "Z- factor," is defined, which is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment.
Tackling drug-resistant infections globally: final report and recommendations
TL;DR: Suggested reading taken from the last 12 months of the Commission’s weekly publication “On the Radar” highlights papers and reporting exploring the evidence for the effectiveness of antimicrobial stewardship in hospitals, the scope of the problem of antimacterial resistance, and some specific stewardship strategies.
Journal ArticleDOI
The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria
Rudolph Hugh,Einar Leifson +1 more
TL;DR: In the absence of compounds such as nitrates, the oxidation of carbohydrates is a strictly aerobic process, whereas fermentation is an anaerobic process.
Journal ArticleDOI
Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium.
L. A. Collins,Scott G. Franzblau +1 more
TL;DR: MABA is sensitive, rapid, inexpensive, and nonradiometric and offers the potential for screening, with or without analytical instrumentation, large numbers of antimicrobial compounds against slow-growing mycobacteria.
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Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling.
TL;DR: A model in which M. tuberculosis arrests growth, decreases its respiration rate and is resistant to isoniazid, rifampicin and metronidazole is established, which is generated a model with which to search for agents active against persistent M.culosis.