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Molecular genetics of yeast :a practical approach
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The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly, and the construction and use of cDNA libraries for genetic selections.Abstract:
1: Procedures for isolating yeast DNA for different purposes. 2: Construction of cloning and expression vectors. 3: Cosmid cloning of yeast DNA. 4: The construction and use of cDNA libraries for genetic selections. 5: Pulsed field gel electrophoresis. 6: Plasmid shuffling and mutant isolation. 7: Ty insertional mutagenesis. 8: High efficiency transformation with lithium acetate. 9: Measurement of transcription. 10: Measurement of mRNA stability. 11: Production of foreign proteins at high level. 12: Cell-free translation of natural and synthetic mRNAs. 13: Virus-like particles: Ty retrotransposons. 14: The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly. 15: Immuno-electron microscopy. 16: Industrial Saccharomyces yeastsread more
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Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway
TL;DR: Analysis of viable null alleles revealed that Mec1 plays a greater role in response to inhibition of DNA synthesis than Rad53, suggesting that this checkpoint pathway plays an important role in the maintenance of DNA synthetic capabilities when DNA replication is stressed.
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The Crystal Structure of Human Eukaryotic Release Factor eRF1—Mechanism of Stop Codon Recognition and Peptidyl-tRNA Hydrolysis
Haiwei Song,Haiwei Song,Pierre M. Mugnier,Amit Das,Helen M. Webb,David R. H. Evans,Mick F. Tuite,Brian A. Hemmings,David Barford,David Barford +9 more
TL;DR: The release factor eRF1 terminates protein biosynthesis by recognizing stop codons at the A site of the ribosome and stimulating peptidyl-tRNA bond hydrolysis at the peptidol transferase center, revealing the molecular mechanism of release factor activity.
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The Pdr12 ABC transporter is required for the development of weak organic acid resistance in yeast
Peter W. Piper,Yannick Mahé,Suzanne Thompson,Rudy Pandjaitan,Caroline D. Holyoak,Ralf Egner,Manuela Mühlbauer,Peter J. Coote,Karl Kuchler +8 more
TL;DR: This is the first demonstration that regulated expression of a eukaryotic ABC transporter mediates weak organic acid resistance development, the cause of widespread food spoilage by yeasts, and the data suggest that the inhibition of this transporter could be a strategy for preventingFood spoilage.
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Genome-Wide Analysis of mRNA Stability Using Transcription Inhibitors and Microarrays Reveals Posttranscriptional Control of Ribosome Biogenesis Factors
TL;DR: This examination showed that Ccr4p, the major yeast mRNA deadenylase, contributes to the degradation of transcripts encoding both ribosomal proteins and rRNA synthesis and ribosome assembly factors and mediates a large part of the transcriptional response to heat stress.
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Functional activity and role of cation-efflux family members in Ni hyperaccumulation in Thlaspi goesingense
TL;DR: It is proposed that the high-level expression of TgMTP1 in T. goesingense accounts for the enhanced ability of this hyperaccumulator to accumulate metal ions within shoot vacuoles.