Journal ArticleDOI
Molecular profile of African swine fever virus (ASFV) circulating in Vietnam during 2019-2020 outbreaks.
Nguyen Tuan Anh Mai,Xuan Dang Vu,Thi Thu Huyen Nguyen,Van Tam Nguyen,Thi Bich Ngoc Trinh,Yong Joo Kim,Hyun-Joo Kim,Ki-Hyun Cho,Thi Lan Nguyen,Thi Nga Bui,Dae Gwin Jeong,Sun-Woo Yoon,Thang Truong,Aruna Ambagala,Daesub Song,Van Phan Le +15 more
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TLDR
The first intra-epidemic genome analysis reported for the ASFV strains circulating in Vietnam was reported in 2019-2020 by Nguyen et al. as mentioned in this paper, who determined DNA sequences for a portion of the B646L (p72) gene, the complete E183L gene, variable region of EP402R (CD2v), the central variable region (CVR) of pB602L, and a tandem repeat sequence (TRS) between the I73R and I329L genes.Abstract:
African swine fever (ASF) is a highly infectious disease of pigs caused by African swine fever virus (ASFV). In order to identify potential genetic variations among ASFV strains circulating in Vietnam, 26 ASFV isolates from organs and blood samples collected from domestic pigs from 23 different provinces of northern, central and southern Vietnam during 2019-2020 ASF outbreaks were genetically characterized. Nucleotide sequences were determined for a portion of the B646L (p72) gene, the complete E183L (p54) gene, the variable region of EP402R (CD2v), the central variable region (CVR) of pB602L, and a tandem repeat sequence (TRS) between the I73R and I329L genes. Analysis of the partial B646L (p72) and EP402R (CD2v) gene sequences and the full-length E183L (p54) gene sequence showed that all 26 ASFV isolates belonged to genotype II and serotype VIII and that they were identical to the strain Georgia/2007/1 and all ASFV strains sequenced in China. The TRS between the I73R and I329L genes contained a 10-nucleotide insertion that was observed in the Chinese ASFV strain CN201801 isolated from domestic pigs in 2018, but not in the Georgia/2007/1 and China/Jilin/2018/boar strains isolated from wild boar in China. This is the first intra-epidemic genome analysis reported for the ASFV strains circulating in Vietnam.read more
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Journal ArticleDOI
Dynamics of African swine fever virus (ASFV) infection in domestic pigs infected with virulent, moderate virulent and attenuated genotype II ASFV European isolates
Carmina Gallardo,Alejandro Soler,Imbi Nurmoja,Cristina Cano-Gómez,Svetlana Cvetkova,Maciej Frant,Grzegorz Woźniakowski,A. Simón,Covadonga Pérez,Raquel Nieto,Marisa Arias +10 more
TL;DR: In this paper, the authors compared the infection dynamics of three genotype II African swine fever viruses (ASFV) circulating in Europe, and provided quantitative data on the spread and excretion of different virulence among domestic pigs that can help to better focus surveillance activities and, thus, increase the ability to detect ASF introductions earlier.
Journal ArticleDOI
Pathogenicity of an African swine fever virus strain isolated in Vietnam and alternative diagnostic specimens for early detection of viral infection.
Hu Suk Lee,Vuong Nghia Bui,Duy Tung Dao,Ngoc Anh Bui,Thanh Le,Minh Anh Kieu,Quang Huy Nguyen,Long Hoang Tran,Jae-Hee Roh,Kyoung-Min So,Tai-Young Hur,Sang-Ik Oh +11 more
TL;DR: In this paper, the authors investigated the pathogenicity of ASFV and explored alternative sampling methods for early detection, such as chewed rope-based oral fluid collection and ear tissue samples.
Journal ArticleDOI
Multiple variants of African swine fever virus circulating in Vietnam
Van Tam Nguyen,Ki-Hyun Cho,Nguyen Tuan Anh Mai,Jee-Yong Park,Thi Bich Ngoc Trinh,Min-Kyung Jang,Thi Thu Huyen Nguyen,Xuan Dang Vu,Thi Lan Nguyen,Van Diep Nguyen,Aruna P. N. Ambagala,Yong-Joo Kim,Van Phan Le +12 more
Journal ArticleDOI
Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China
TL;DR: The results indicated that ASFV strains circulating in Guangxi province during 2019–2020 outbreaks showed high genetic diversity, of which genotypes I and II, as well as serogroups 4 and 8, were simultaneously circulating in China, and there existed wild-type and naturally gene-deleted strains in the field.
Journal ArticleDOI
African Swine Fever Virus K205R Induces ER Stress and Consequently Activates Autophagy and the NF-κB Signaling Pathway
Qi Wang,Luyu Zhou,Jiang Wang,Dan Su,Dahua Li,Yong-Kun Du,Guo-Yu Yang,Gaiping Zhang,Bei-Bei Chu +8 more
TL;DR: A previously uncharacterized role of ASFV K205R in endoplasmic reticulum stress, autophagy, and the NF-κB signaling pathway is demonstrated.
References
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Journal ArticleDOI
African swine fever virus replication and genomics
TL;DR: African swine fever virus (ASFV) is a large icosahedral DNA virus which replicates predominantly in the cytoplasm of infected cells and a similar mechanism of replication to Poxviruses has been proposed for ASFV.
Journal ArticleDOI
Genotyping field strains of African swine fever virus by partial p72 gene characterisation.
Armanda D.S. Bastos,Marie-Louise Penrith,Catherine Crucière,J.L. Edrich,Geoff Hutchings,François Roger,Emmanuel Couacy-Hymann,G. R. Thomson +7 more
TL;DR: A PCR-based sequencing method was developed which permits detection and characterization of African swine fever virus (ASFV) variants within 5 and 48 h, respectively, of receipt of a clinical specimen, useful for molecular epidemiological clarification of ASF.
Journal ArticleDOI
African swine fever: A re-emerging viral disease threatening the global pig industry
TL;DR: Genotype II African swine fever virus (ASFV) circulating in Europe has high pathogenicity for domestic pigs and wild boar and relies on ‘stamping-out’ policies and control of pig movements, which have a significant impact on affected regions.
Journal ArticleDOI
Molecular Characterization of African Swine Fever Virus, China, 2018.
Shengqiang Ge,Jinming Li,Fan Xiaoxu,Fuxiao Liu,Lin Li,Qinghua Wang,Weijie Ren,Jingyue Bao,Chunju Liu,Wang Hua,Yutian Liu,Yongqiang Zhang,Tiangang Xu,Xiaodong Wu,Zhiliang Wang +14 more
TL;DR: A virus from an African swine fever–positive pig sample was subjected to phylogenetic analysis and showed that the causative strain belonged to the p72 genotype II and CD2v serogroup 8.