Molecular screening of 246 Portuguese Aspergillus isolates among different clinical and environmental sources
Raquel Sabino,Cristina Veríssimo,Helena Parada,João Brandão,Carla Viegas,Elisabete Carolino,Karl V. Clemons,David A. Stevens,David A. Stevens +8 more
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TLDR
Knowing the species-complex epidemiology for each setting, as well as the identification of cryptic species among the collected clinical isolates, is important to allow preventive and corrective measures to be taken, which may result in decreased exposure to those organisms and a better prognosis.Abstract:
Clinical and environmental samples from Portugal were screened for the presence of Aspergillus and the distributions of the species complexes were determined in order to understand how their distributions differ based on their source. Fifty-seven Aspergillus isolates from clinical samples were collected from 10 health institutions. Six species complexes were detected by internal transcribed spacer sequencing; Fumigati, Flavi ,a nd Nigri were found most frequently (50.9%, 21.0%, and 15.8%, respectively). β-tubulin and calmodulin sequencing resulted in seven cryptic species (A. awamorii, A. brasiliensis, A. fructus, A. lentulus, A. sydowii, A. tubingensis, Emericella echinulata) being identified among the 57 isolates. Thirty-nine isolates of Aspergillus were recovered from beach sand and poultry farms, 31 from swine farms, and 80 from hospital environments, for a total 189 isolates. Eleven species complexes were found in these 189 isolates, and those belonging to the Versicolores species complex were found most frequently (23.8%). There was a significant association between the different environmental sources and distribution of the species complexes; the hospital environment had greater variability of species complexes than other environmental locations. A high prevalence of cryptic species within the Circumdati complex was detected in several environments; from the isolates analyzed, at least four cryptic species were identified, most of them growing at 37 o C. Because Aspergillus species complexes have different susceptibilities to antifungals, knowing the species-complex epidemiology for each setting, as well as the identification of cryptic species among the collected clinical isolates, is important. Thisread more
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Beach sand and the potential for infectious disease transmission: observations and recommendations
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TL;DR: The literature on pathogen levels in beach sand, and their potential for affecting human health, is reviewed, to provide specific recommendations for sand sampling programmes and to provide background on spatial distribution and temporal characteristics of microbes in sand.
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Inhibition of Aspergillus fumigatus and Its Biofilm by Pseudomonas aeruginosa Is Dependent on the Source, Phenotype and Growth Conditions of the Bacterium
Jose A. G. Ferreira,John C. Penner,Richard B. Moss,Janus A. J. Haagensen,Karl V. Clemons,Alfred M. Spormann,Hasan Nazik,Kevin Cohen,Niaz Banaei,Elisabete Carolino,David A. Stevens +10 more
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