Molecular Weight Determination of Sendai and Newcastle Disease Virus RNA
Daniel Kolakofsky,Daniel Kolakofsky,Edward Boy de la Tour,Edward Boy de la Tour,Hajo Delius,Hajo Delius +5 more
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TLDR
It would appear that the conditions of denaturation used do not equally denature parainfluenza virus RNA and other RNAs, such as cellular rRNA, 45S rRNA precursor, and R17 RNA.Abstract:
The molecular weights of Sendai and Newcastle disease virus RNA were estimated by sedimentation in sucrose gradients and by length measurements in the electron microscope under both denaturing and nondenaturing conditions. Sedimentation analyses under denaturing conditions yielded molecular weight estimates of 2.3 x 10(6) to 2.6 x 10(6), whereas length measurements yielded estimates of 5.2 x 10(6) to 5.6 x 10(6) for both denatured and nondenatured viral RNA. It would appear that the conditions of denaturation used (99% dimethyl sulfoxide at 26 C, and reaction with 1.1 M formaldehyde for 10 min at 60 C) do not equally denature parainfluenza virus RNA and other RNAs, such as cellular rRNA, 45S rRNA precursor, and R17 RNA.read more
Citations
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Book ChapterDOI
Reproduction of Paramyxoviruses
TL;DR: Measles, canine distemper, and rinderpest viruses form a distinct subgroup on the basis of antigenicity, hemagglutinating characteristics, and lack of evidence for a virion-associated neuraminidase or neuraminic acid-containing cellular receptors, but it is now generally accepted that these viruses should be included in the paramyxovirus group because of their similar structural properties.
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Isolation and characterization of Sendai virus DI-RNAs.
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Sendai virus contains overlapping genes expressed from a single mRNA.
TL;DR: Sequence homologies at the beginning of three Sendai virus cistrons suggest that these genes may have arisen by duplication from a common ancestor, possibly an influenza-like virus gene.
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Ribonucleoproteins of Uukuniemi virus are circular.
TL;DR: The internal ribonucleoprotein (RNP) of Uukuniemi virus was released with Triton X-100 and analyzed on sucrose gradients and showed that all three species were circular.
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Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels.
TL;DR: Based on absolute molecular weight standards, obtained from sequenced rRNA of Escherichia coli and tRNA and extrapolating to higher molecular weights the size of animal cell was reexamined and seems to represent the largest size of non-viral animal-cell RNA molecules.
References
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Journal ArticleDOI
Electrophoretic separation of viral nucleic acids on polyacrylamide gels
TL;DR: The elution of viral RNA from gel slices and the demonstration of infectivity after electrophoresis are described, showing a general relationship between the logarithm of the molecular weight and the relative electrophoretic mobility.
Journal ArticleDOI
Partial denaturation of thymine- and 5-bromouracil-containing λ DNA in alkali
Ross B. Inman,Maria Schnös +1 more
TL;DR: An electron microscopic examination of λ DNA, partially denatured at high pH, shows that the sections which denature first are at the same positions as those previously observed for λDNA partially den atured by heat.
Journal ArticleDOI
Some physical properties of bacteriophage r17 and its ribonucleic acid.
TL;DR: The solution conformation of bacteriophage RNA is more compact than that of other ribonucleic acids studied to date, however, there is no evidence that this tighter packing reflects an unusual secondary structure.
Journal ArticleDOI
Processing of 45 s nucleolar RNA.
TL;DR: The present data suggest that 36 s and 24 s molecules may be generated when the order of cleavage steps is altered, although their possible role in normal processing cannot be completely excluded.