Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering
Chen Ling,George L Peabody,Davinia Salvachúa,Young-Mo Kim,Colin M Kneucker,Christopher H. Calvey,Michela A Monninger,Nathalie Muñoz,Brenton C. Poirier,Kelsey J. Ramirez,Peter C. St. John,Sean P. Woodworth,Jon K. Magnuson,Kristin E. Burnum-Johnson,Adam M. Guss,Christopher W. Johnson,Gregg T. Beckham +16 more
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In this paper , Pseudomonas putida KT2440 is engineered to convert glucose and xylose, the primary carbohydrates in lignocellulosic hydrolysates, to muconic acid using a model-guided strategy to maximize the theoretical yield.Abstract:
Muconic acid is a bioprivileged molecule that can be converted into direct replacement chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this study, Pseudomonas putida KT2440 is engineered to convert glucose and xylose, the primary carbohydrates in lignocellulosic hydrolysates, to muconic acid using a model-guided strategy to maximize the theoretical yield. Using adaptive laboratory evolution (ALE) and metabolic engineering in a strain engineered to express the D-xylose isomerase pathway, we demonstrate that mutations in the heterologous D-xylose:H+ symporter (XylE), increased expression of a major facilitator superfamily transporter (PP_2569), and overexpression of aroB encoding the native 3-dehydroquinate synthase, enable efficient muconic acid production from glucose and xylose simultaneously. Using the rationally engineered strain, we produce 33.7 g L-1 muconate at 0.18 g L-1 h-1 and a 46% molar yield (92% of the maximum theoretical yield). This engineering strategy is promising for the production of other shikimate pathway-derived compounds from lignocellulosic sugars. read more
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Revealing Pentose Catabolism in Pseudomonas putida
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