Open Access
Multifocal multiphoton microscopy : new detection methods and biological applications
Reads0
Chats0
About:
The article was published on 2006-01-01 and is currently open access. It has received 8 citations till now. The article focuses on the topics: Multifocal multiphoton microscopy.read more
Citations
More filters
Journal Article
High-resolution nonlinear optical imaging of live cells by second harmonic generation
TL;DR: It was found that chirality plays a significant role in the mechanism of contrast generation, and it is also shown that SHIM is highly sensitive to membrane potential, with a depolarization of 25 mV resulting in an approximately twofold loss of signal intensity.
Journal Article
Comparison of three-dimensional imaging properties between two-photon and single-photon fluorescence microscopy
Min Gu,Colin J. R. Sheppard +1 more
TL;DR: In this article, the imaging performance in single-photon (l-p) and two-p (2p) fluorescence microscopy is compared in terms of the three-dimensional (3D) point spread function and the 3-D optical transfer function.
Journal Article
Multiphoton fluorescence excitation: New spectral windows for biological nonlinear microscopy
TL;DR: The measured nonlinear excitation spectra and their photophysical characteristics that empower nonlinear laser microscopy for biological imaging are described.
Journal Article
Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm
TL;DR: F fluorophore fluorescence intensities showed no significant departure from quadratic illumination power dependence, indicating genuine two-photon processes, and implications for two- photon laser-scanning microscopy are discussed.
Journal Article
Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin
TL;DR: In this article, the authors used multiphoton excitation microscopy at 730 nm and 960 nm to image in vivo human skin autofluorescence from the surface to a depth of approximately 200 microm.
References
More filters
Book
Principles of Optics
TL;DR: In this paper, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Book
The Principles of Nonlinear Optics
TL;DR: In this article, the authors present a general description of wave propagation in nonlinear media, including high-resolution nonlinear optical spectroscopy, and four-wave mixing and mixing.
Journal ArticleDOI
Deep tissue two-photon microscopy
Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.
Journal ArticleDOI
Nonlinear magic: multiphoton microscopy in the biosciences
TL;DR: Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.