Journal ArticleDOI
Nanotechnology to advance CRISPR-Cas genetic engineering of plants.
Gozde S. Demirer,Gozde S. Demirer,Tallyta N. Silva,Tallyta N. Silva,Christopher T. Jackson,Jason B. Thomas,David W. Ehrhardt,Seung Y. Rhee,Jenny C. Mortimer,Jenny C. Mortimer,Jenny C. Mortimer,Markita P. Landry +11 more
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TLDR
In this article, the authors identify major barriers preventing CRISPR-mediated plant genetic engineering from reaching its full potential, and discuss ways that nanoparticle technologies can lower or eliminate these barriers.Abstract:
CRISPR-Cas genetic engineering of plants holds tremendous potential for providing food security, battling biotic and abiotic crop stresses caused by climate change, and for environmental remediation and sustainability. Since the discovery of CRISPR-Cas technology, its usefulness has been demonstrated widely, including for genome editing in plants. Despite the revolutionary nature of genome-editing tools and the notable progress that these tools have enabled in plant genetic engineering, there remain many challenges for CRISPR applications in plant biotechnology. Nanomaterials could address some of the most critical challenges of CRISPR genome editing in plants through improvements in cargo delivery, species independence, germline transformation and gene editing efficiency. This Perspective identifies major barriers preventing CRISPR-mediated plant genetic engineering from reaching its full potential, and discusses ways that nanoparticle technologies can lower or eliminate these barriers. We also describe advances that are needed in nanotechnology to facilitate and accelerate plant genome editing. Timely advancement of the application of CRISPR technologies in plant engineering is crucial for our ability to feed and sustain the growing human population under a changing global climate.read more
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Melatonin-mediated temperature stress tolerance in plants
Ali Raza,Sidra Charagh,Pedro García-Caparrós,Md. Atikur Rahman,Vincent Happy Ogwugwa,Faisal Iqbal Saeed,Wanmei Jin +6 more
TL;DR: In this paper , the critical role of MET in plant production and tolerance against temperature stress is highlighted, and how MET interacts with other molecules like phytohormones and gaseous molecules to alleviate temperature stress.
Journal ArticleDOI
Recent advances in nano-enabled agriculture for improving plant performance
TL;DR: The aim in this review is to aid researchers to learn quickly how to use plant nanobiotechnology for improving agricultural production and to discuss the knowledge gaps in these topics.
Journal ArticleDOI
Recent advances in nano-enabled agriculture for improving plant performance
TL;DR: In this paper , a review of the application of plant nanobiotechnology in agriculture is presented, focusing on the following topics: improving stress tolerance, early detection, targeted delivery and controlled release of agrochemicals, transgenic events in non-model crop species, and seed nanopriming.
Journal ArticleDOI
Antioxidants as modulators of arsenic-induced oxidative stress tolerance in plants: An overview.
Faisal Zulfiqar,Muhammad Ashraf +1 more
TL;DR: In this article, the authors consider how different types of antioxidants participate in the oxidative defense mechanism to alleviate As stress in plants and discuss the potential for "Omics" technologies to uncover molecular mechanisms.
Journal ArticleDOI
Nanotechnology Strategies for Plant Genetic Engineering
TL;DR: In this paper , the progress in the development of nanomaterial-based gene-delivery systems is considered, and the conceptual innovations, methods, and practical applications of nanomedicine-mediated genetic transformation summarized herein will be beneficial for promoting plant genetic engineering in modern agriculture.
References
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Journal ArticleDOI
DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu,David A. Scott,David A. Scott,Joshua A. Weinstein,Joshua A. Weinstein,F. Ann Ran,F. Ann Ran,F. Ann Ran,Silvana Konermann,Silvana Konermann,Vineeta Agarwala,Vineeta Agarwala,Vineeta Agarwala,Yinqing Li,Yinqing Li,Eli J. Fine,Xuebing Wu,Ophir Shalem,Ophir Shalem,Thomas J. Cradick,Luciano A. Marraffini,Gang Bao,Feng Zhang,Feng Zhang +23 more
TL;DR: In this article, the Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing.
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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Alexis C. Komor,Yongjoo Kim,Yongjoo Kim,Michael S. Packer,Michael S. Packer,John A. Zuris,John A. Zuris,David R. Liu,David R. Liu +8 more
TL;DR: E engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution.
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RNA-programmed genome editing in human cells
TL;DR: It is shown here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA breaks at a site complementary to the guide RNA sequence in genomic DNA.
Journal ArticleDOI
Mesoporous silica nanoparticles deliver DNA and chemicals into plants
TL;DR: A honeycomb mesoporous silica nanoparticle system with 3-nm pores that can transport DNA and chemicals into isolated plant cells and intact leaves and capped the ends with gold nanoparticles to keep the molecules from leaching out is shown.
Journal ArticleDOI
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Je Wook Woo,Jung-Eun Kim,Soon Il Kwon,Claudia Corvalán,Seung Woo Cho,Hyeran Kim,Sang-Gyu Kim,Sangtae Kim,Sunghwa Choe,Sunghwa Choe,Jin-Soo Kim +10 more
TL;DR: Transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%.