Journal ArticleDOI
Necrotizing meningoencephalitis of Pug Dogs associates with dog leukocyte antigen class II and resembles acute variant forms of multiple sclerosis
Kimberly A. Greer,Aaron K. Wong,Hongwei Liu,Thomas R. Famula,Niels C Pedersen,Alison L. Ruhe,M. Wallace,Mark W. Neff +7 more
Reads0
Chats0
TLDR
P Pug Dog encephalitis (PDE) shares clinical features with the less common acute variant forms of MS, and NME of Pug Dogs may represent a naturally occurring canine model of certain idiopathic inflammatory disorders of the human central nervous system.Abstract:
Necrotizing meningoencephalitis (NME) is a disorder of Pug Dogs that appears to have an immune etiology and high heritability based on population studies. The present study was undertaken to identify a genetic basis for the disease. A genome-wide association scan with single tandem repeat (STR) markers showed a single strong association near the dog leukocyte antigen (DLA) complex on CFA12. Fine resolution mapping with 27 STR markers on CFA12 further narrowed association to the region containing DLA-DRB1, -DQA1 and, -DQB1 genes. Sequencing confirmed that affected dogs were more likely to be homozygous for specific alleles at each locus and that these alleles were linked, forming a single high risk haplotype. The strong DLA class II association of NME in Pug Dogs resembles that of human multiple sclerosis (MS). Like MS, NME appears to have an autoimmune basis, involves genetic and nongenetic factors, has a relatively low incidence, is more frequent in females than males, and is associated with a vascularly orientated nonsuppurative inflammation. However, NME of Pug Dogs is more aggressive in disease course than classical human MS, appears to be relatively earlier in onset, and involves necrosis rather than demyelination as the central pathobiologic feature. Thus, Pug Dog encephalitis (PDE) shares clinical features with the less common acute variant forms of MS. Accordingly, NME of Pug Dogs may represent a naturally occurring canine model of certain idiopathic inflammatory disorders of the human central nervous system.read more
Citations
More filters
Journal ArticleDOI
Perspectives on meningoencephalomyelitis of unknown origin.
Joan R. Coates,Nick D. Jeffery +1 more
TL;DR: The current understanding of MUO and its phenotypic variants encountered in clinical practice is highlighted and a more targeted treatment approach is becoming attainable.
Journal ArticleDOI
Clinical presentation, diagnostic findings, prognostic factors, treatment and outcome in dogs with meningoencephalomyelitis of unknown origin: A review.
TL;DR: An overview of recent developments in the clinical presentation, diagnostic findings, possible prognostic factors, treatment and outcome in dogs diagnosed with MUO is provided.
Journal ArticleDOI
Broadly reactive polymerase chain reaction for pathogen detection in canine granulomatous meningoencephalomyelitis and necrotizing meningoencephalitis.
Renee M. Barber,Brian F. Porter,Qiang Li,Meghan May,Mary Kate Claiborne,A.B. Allison,Elizabeth W. Howerth,A. Butler,S. Wei,Jonathan M. Levine,Gwendolyn J. Levine,Daniel R. Brown,Scott J. Schatzberg +12 more
TL;DR: The negative results suggest that viral pathogens are not common in the brain tissue of dogs with GME and NME.
Journal ArticleDOI
Identification of Risk Loci for Necrotizing Meningoencephalitis in Pug Dogs
Renee M. Barber,Scott J. Schatzberg,Jason J. Corneveaux,April N. Allen,Brian F. Porter,Jeremy J. Pruzin,Simon R. Platt,Marc Kent,Matthew J. Huentelman +8 more
TL;DR: The results support the utility of this high-density canine whole-genome array, confirm that dogs are a powerful model for mapping complex genetic disorders and provide important preliminary data to support in depth genetic analysis of NME in numerous affected breeds.
Journal ArticleDOI
Comprehensive immunohistochemical studies on canine necrotizing meningoencephalitis (NME), necrotizing leukoencephalitis (NLE), and granulomatous meningoencephalomyelitis (GME).
TL;DR: The histopathology of the lesions caused by these conditions and the inflammatory cell populations produced in response to them were examined among dogs affected with GME, NME, or NLE to understand their pathogeneses.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Heterogeneity of multiple sclerosis lesions: implications for the pathogenesis of demyelination.
Claudia F. Lucchinetti,Wolfgang Brück,Joseph E. Parisi,Bernd W. Scheithauer,Moses Rodriguez,Hans Lassmann +5 more
TL;DR: At a given time point of the disease, the patterns of demyelination were heterogeneous between patients, but were homogenous within multiple active lesions from the same patient, suggesting that MS may be a disease with heterogeneous pathogenetic mechanisms.
Journal ArticleDOI
Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping.
TL;DR: It was clear, however, that features of the template in addition to the 3' terminal base also affect the fraction of product adenylated, and it proved difficult to identify a single sequence capable of protecting the products of all markers from non-templated addition of nucleotide.
Journal ArticleDOI
Permutation Tests for Multiple Loci Affecting a Quantitative Character
TL;DR: Two extensions of the permutation-based method for estimating empirical threshold values are presented, which yield critical values that can be used to construct tests for the presence of minor QTL effects while accounting for effects of known major QTL.
Journal ArticleDOI
Genetics of multiple sclerosis
TL;DR: To overcome the confounding effects of genetic complexity, MS genetic research needs to take advantage of clinical and epidemiological information to better homogenize study samples and shed some light on the mild to moderate susceptibility loci affecting the MS patient population.