Journal ArticleDOI
Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus.
Sheila Ons,Marcos Sterkel,Luis Aníbal Diambra,Henning Urlaub,Rolando Rivera-Pomar,Rolando Rivera-Pomar +5 more
TLDR
A straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry is shown to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector.Abstract:
We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.read more
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Transcriptome analysis of neuropeptides and G-protein coupled receptors (GPCRs) for neuropeptides in the brown planthopper Nilaparvata lugens.
TL;DR: The genes encoding neuropeptides, neurohormones and their putative G-protein coupled receptors were identified in the brown planthopper, Nilaparvata lugens by transcriptome analysis (RNA-seq) and demonstrate the power of RNA-seq as a tool for analyzing the neuropeptic-related genes in the absence of whole genome sequence information.
Journal ArticleDOI
The pleiotropic allatoregulatory neuropeptides and their receptors: A mini-review.
Heleen Verlinden,Marijke Gijbels,Els Lismont,Cynthia Lenaerts,Jozef Vanden Broeck,Elisabeth Marchal +5 more
TL;DR: An overview of allatotropins and allatostatins, and their recently characterized GPCRs are given with a view to better understand their modes of action and different action sites.
Journal ArticleDOI
The Role of Peptide Hormones in Insect Lipid Metabolism
TL;DR: Peptide hormones are also involved in lipid metabolism during reproduction, flight, diapause, starvation, infections and immunity, and the potential of lipid metabolism-related peptide hormones in pest management is discussed.
Journal ArticleDOI
Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
Senne Dillen,Sven Zels,Heleen Verlinden,Jornt Spit,Pieter Van Wielendaele,Jozef Vanden Broeck +5 more
TL;DR: This is the first comprehensive study in which a clearly negative correlation is described between the sNPF signalling pathway and feeding, prompting a reconsideration of the diverse roles of sNPFs in the physiology of insects.
Journal ArticleDOI
Identification of G protein coupled receptors for opsines and neurohormones in Rhodnius prolixus. Genomic and transcriptomic analysis.
Sheila Ons,Andrés Lavore,Marcos Sterkel,Juan Pedro Wulff,Ivana Sierra,Jesús Martínez-Barnetche,Mario H. Rodriguez,Rolando Rivera-Pomar,Rolando Rivera-Pomar +8 more
TL;DR: A genomic analysis and transcriptomic analysis of the R. prolixus central nervous system reveal a high degree of sequence conservation in the molecular components of the neuroendocrine system of triatomines.
References
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Journal ArticleDOI
Improved Prediction of Signal Peptides: SignalP 3.0
TL;DR: Improvements of the currently most popular method for prediction of classically secreted proteins, SignalP, which consists of two different predictors based on neural network and hidden Markov model algorithms, where both components have been updated.
Journal ArticleDOI
Neuropeptides and neuropeptide receptors in the Drosophila melanogaster genome.
Randall S. Hewes,Paul H. Taghert +1 more
TL;DR: The recently completed Drosophila genome sequence for G protein-coupled receptors sensitive to bioactive peptides (peptide GPCRs) is scanned and 44 genes are described that represent the vast majority, and perhaps all, of the peptide G PCRs encoded in the fly genome.
Journal ArticleDOI
Neuropeptides in the nervous system of Drosophila and other insects: multiple roles as neuromodulators and neurohormones.
TL;DR: Drosophila, in spite of its small size, is now emerging as a very favorable organism for the studies of neuropeptide function due to the arsenal of molecular genetics methods available.
Journal ArticleDOI
Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum
Bin Li,Reinhard Predel,Susanne Neupert,Frank Hauser,Yoshiaki Tanaka,Giuseppe Cazzamali,Michael Williamson,Yasuyuki Arakane,Peter Verleyen,Liliane Schoofs,Joachim Schachtner,Cornelis J. P. Grimmelikhuijzen,Yoonseong Park +12 more
TL;DR: The authors' analysis of Tribolium indicates that, during insect evolution, genes for neuropeptides and protein hormones are often duplicated or lost.
Journal ArticleDOI
From the Genome to the Proteome: Uncovering Peptides in the Apis Brain
Amanda B. Hummon,T.A. Richmond,Peter Verleyen,Geert Baggerman,Jurgen Huybrechts,Michael A. Ewing,Evy Vierstraete,Sandra L. Rodriguez-Zas,Liliane Schoofs,Gene E. Robinson,Jonathan V. Sweedler +10 more
TL;DR: This study lays the groundwork for future molecular studies of Apis neuropeptides with the identification of 36 genes, 33 of which were previously unreported, and inferred more than 200 peptides within the Apis mellifera genome.