Journal ArticleDOI
New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat.
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TLDR
Subcloning of a clone of the 120-bp family of rye, pSc119, has produced two extremely useful probes that assays similar repetitive sequence families in both wheat and rye chromosomes.Abstract:
Subcloning of a clone of the 120-bp family of rye, pSc119, has produced two extremely useful probes. pSc119.1 assays rye-specific dispersed repetitive sequence families. It is present on all seven ...read more
Citations
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Journal ArticleDOI
B-chromosome evolution.
TL;DR: Because B chromosomes interact with the standard chromosomes, they can play an important role in genome evolution and may be useful for studying molecular evolutionary processes.
Journal ArticleDOI
Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes.
TL;DR: Chinese Spring wheat showed that the distal 32% of the long arm was derived from a B genome chromosome, and by using two highly repeated sequence probes, pSc 119.2 and pAsl, and two fluorochromes simultaneously, the B and D genome chromosomes and chromosomes 1A, 4A, and 5A of wheat were identified.
Journal ArticleDOI
The large-scale genomic organization of repetitive DNA families at the telomeres of rye chromosomes.
TL;DR: It is suggested that repetitive DNA sequences in the terminal heterochromatin of rye chromosomes define a basic higher order structure and DNA loop domains of regions of Rye chromosomes consisting of arrays of tandemly organized sequences.
Journal ArticleDOI
Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat–rye addition lines
TL;DR: Flow cytometry was proven to be sensitive enough to detect the small DNA content deviations that are expected to occur in aneuploid plants of wheat.
Journal ArticleDOI
Identification of the entire chromosome complement of bread wheat by two-colour FISH.
Carsten Pedersen,Peter Langridge +1 more
TL;DR: Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
A molecular description of telomeric heterochromatin in Secale species.
TL;DR: The physical properties, sequence divergence and chromosomal distribution of six different repeated sequences in Secale cereale (cultivated rye) are described and it is suggested that each of the S. cereale-specific repeats may have evolved by the insertion of DNA elements into an array of simple repeats followed by amplification of the portion of the array containing the inserted sequence.
Journal ArticleDOI
Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes
A. L. Rayburn,Bikram S. Gill +1 more
TL;DR: Biotin labeling was found to be a rapid, consistent, and reliable technique to detect repeated DNA sequences by in situ hybridization in wheat and should be a useful technique for the physical mapping of DNA sequences on plant chromo- somes.
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A molecular description of telomeric heterochromatin in Secale species.
Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes.
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A. Lane Rayburn,Bikram S. Gill +1 more