Nicotinic receptor-mediated catecholamine secretion from individual chromaffin cells. Chemical evidence for exocytosis.
David J. Leszczyszyn,Jeffrey A. Jankowski,O H Viveros,E J Diliberto,Joseph A. Near,R. M. Wightman +5 more
TLDR
Nicotinic receptor-mediated secretion of catecholamines from individual cultured bovine adrenal medullary chromaffin cells was measured and characterized with a voltametric microelectrode placed adjacent to the cells, consistent with direct chemical measurement of single exocytotic events.About:
This article is published in Journal of Biological Chemistry.The article was published on 1990-09-05 and is currently open access. It has received 231 citations till now. The article focuses on the topics: Chromaffin cell & Adrenal medulla.read more
Citations
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Journal ArticleDOI
Secretory Granule Exocytosis
Robert D. Burgoyne,Alan Morgan +1 more
TL;DR: The wide range of cell types in which regulated secretory granule exocytosis occurs is described and the evidence for the expression of the conserved fusion machinery in these cells is assessed.
Journal ArticleDOI
Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells
TL;DR: In this article, the authors show that under voltage-clamp conditions, stochastically occurring signals can be recorded from adrenal chromaffin cells using a carbon-fibre electrode as an electrochemical detector.
Journal ArticleDOI
The PC12 cell as model for neurosecretion
TL;DR: It is more than likely that in the following decade PC12 cells will continue to serve as a model to study exocytosis, which has some advantages over other models for neurosecretion, including chromaffin cells.
Journal ArticleDOI
Electrochemical monitoring of single cell secretion: vesicular exocytosis and oxidative stress.
TL;DR: Communication between cellular organisms occurs, among other mechanisms, through the release of specific biochemical or chemicalmessengers by an emitting cell, generally coupled to a specific detection of these messengers by a receiving cell.
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Analysis of exocytotic events recorded by amperometry.
TL;DR: The purpose of this review is to provide practical guidelines for performing amperometric recordings of exocytotic activity and interpreting the results based on shape characteristics of individual release events.
References
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Journal ArticleDOI
Spontaneous release of transmitter from growth cones of embryonic neurones
S. Young,Mu-ming Poo +1 more
TL;DR: It is reported that single-channel activity resembling that of muscle acetylcholine receptor channels was induced when the probe was positioned near the growth cones of 50% of the neurones, suggesting the spontaneous release of acetylCholine (ACh) from these growth cones.
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Acetylcholine release from growth cones detected with patches of acetylcholine receptor-rich membranes.
TL;DR: It is reported here that the growth cones of embryonic chick ciliary ganglion neurones can release acetylcholine, even when the cells are grown in the absence of target myotubes.
Journal ArticleDOI
Vesicle hypothesis of the release of quanta of acetylcholine.
B Ceccarelli,W P Hurlbut +1 more