Journal ArticleDOI
Novel regulation of transcription initiation of the peptide IX gene of adenovirus 2.
TLDR
The upstream element of the pIX gene appears to have a novel function: suppression of the transcriptional repression exerted by a downstream sequence, leading to a net transcription activation.Abstract:
cis-Acting elements involved in transcription of the peptide IX (pIX) gene of adenovirus 2 were identified by using in vivo transient expression assays and two in vitro transcription systems. Deletion of either the sequence between positions -45 and -70 or the TATA box abolished the initiation of pIX gene transcription in vivo and transcription with HeLa cell nuclear extracts in vitro. These results initially suggested the presence of a positive factor acting on the upstream element. However, when proteins in the nuclear extract were fractionated by column chromatography and analyzed by reconstitution of transcription in vitro, it was found that a certain fraction could direct TATA box-dependent transcription initiation even in the absence of the upstream element. Furthermore, activity inhibiting TATA box-dependent transcription was found in the nuclear extract. In contrast, inhibition of TATA box-dependent transcription was suppressed by deletion of a downstream sequence between positions +33 and +122. These results indicate that the TATA box of the pIX gene by itself has the ability to direct initiation of constitutive transcription but that the function of this element is under negative control by a repressor acting on a downstream sequence. Thus, the upstream element of the pIX gene appears to have a novel function: suppression of the transcriptional repression exerted by a downstream sequence, leading to a net transcription activation. Possible mechanisms for transcription initiation of pIX DNA are discussed.read more
Citations
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Journal ArticleDOI
New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses.
Frits Jacobus Fallaux,Abraham Bout,Ietje van der Velde,Diana J. M. van den Wollenberg,Kathleen M. Hehir,Jesse Keegan,Crys Auger,Steve J. Cramer,Hans van Ormondt,Alex J. van der Eb,Dinko Valerio,Rob C. Hoeben +11 more
TL;DR: The combination of PER.C6 cells and nonoverlapping E1-deleted adenoviral vectors eliminates the problem of RCA generation by homologous recombination, and allows cost-effective production of safe, clinical-grade batches of recombinant Ad vectors.
Journal ArticleDOI
Promoter of the adenovirus polypeptide IX gene: similarity to E1B and inactivation by substitution of the simian virus 40 TATA element.
L E Babiss,L D Vales +1 more
TL;DR: Results will be discussed with respect to exchangeability of promoter elements and the possible role of the viral E1B 21-kDa protein in potentiating or stabilizing transcription factor TFIID binding to the pIX TATA element.
Journal ArticleDOI
Profiling the Thermodynamic Softness of Adenoviral Promoters
Chu H. Choi,Zoi Rapti,Zoi Rapti,Vladimir Gelev,Michele R. Hacker,Boian S. Alexandrov,Evelyn J. Park,Jae Suk Park,Nobuo Horikoshi,Augusto Smerzi,Kim Ø. Rasmussen,A. R. Bishop,Anny Usheva +12 more
TL;DR: The results suggest that genetic information is stored not only in explicit codon sequences, but also may be encoded into local dynamic and structural features, and that it may be possible to access this obscured information using DNA dynamics calculations.
Journal ArticleDOI
Tissue-Specific Distribution of a Novel C-Terminal Truncation Retinoic Acid Receptor Mutant Which Acts as a Negative Repressor in a Promoter- and Cell-Type-Specific Manner
T Matsui,S Sashihara +1 more
TL;DR: DNA-binding analysis revealed that RAR gamma Bm382 is able to form a heterodimer with the retinoid X receptor and bind to the different types of retinoic acid response elements with almost the same efficiency as normal RAR.
Journal ArticleDOI
cis-dominant defect in activation of adenovirus type 5 E1b early RNA synthesis.
TL;DR: If the E1a protein-coding region contains cis-acting regulatory sequences, they are not part of the previously mapped E1b transcriptional control region and may represent additional regulatory elements that ensure prompt and efficient E1B expression during the early phase of infection.
References
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Journal ArticleDOI
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
TL;DR: A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Journal ArticleDOI
Regulation of inducible and tissue-specific gene expression
TL;DR: A number of cases of inducible and tissue-specific gene expression involve the activation of preexisting transcription factors, rather than the synthesis of new proteins, which may involve covalent modification of the protein or an allosteric change in its structure.
Journal ArticleDOI
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins
TL;DR: The enzyme that carries out RNA synthesis provides the cell with the means to adjust the patterns of transcription in response to environmental and developmental signals, and the presence of promoter-specific transcription factors enables other components of the transcriptional machinery, including the RNA polymerase, to initiate transcription with selectivity and accuracy.
Journal ArticleDOI
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region
TL;DR: Dissociation rate measurements indicate a cooperative interaction between USF and TFIID when simultaneously bound to the promoter DNA.
Journal ArticleDOI
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract
TL;DR: A cell-free system for studying the synthesis of mRNA in mammalian cells, which consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA.