Proceedings ArticleDOI
Optimal source to detector separation for extracting sub-dermal chromophores in fiber optic diffuse reflectance spectroscopy: a simulation study
TLDR
In this article, the authors used the depth sensitivity of diffuse reflectance spectroscopy and optimal source to detector fiber separation for maximum reflectance collection efficiency from local blood region in skin.Abstract:
Localization and determination of blood region parameters in skin tissue can serve as a valuable supplement to standard non invasive techniques, especially in accessing the degree of depth of burns on skin and for the classification of vascular malformations. Quantitative optical examination of skin local blood region requires the use of depth sensitive techniques and preferential probing for assessment of data from specific layers of skin tissue. This work incorporates the depth sensitivity of diffuse reflectance spectroscopy and optimal source to detector fiber separation for maximum reflectance collection efficiency from local blood region in skin. Monte Carlo simulation for diffuse reflectance was performed on a multi layered skin tissue model consisting of epidermis, perfused dermis and localized blood region. It was found that the slope of the spatially resolved reflectance curve plotted with respect to the source to detector separation distance in semi log scale varies with the depth of the local blood region at specific wavelengths corresponding to the absorption wavelengths of hemoglobin. From the depth information obtained from the spatially resolved reflectance data, the optimum source to detector separation (SDS) is determined for maximum collection efficiency from the chromophore layer. The results obtained from simulation suggest the design of a linearly variable source to detector separation probe for preferential analysis of the depth of a specific tissue layer and subsequent determination of optimal source to detector separation for extracting the layer information.read more
Citations
More filters
Journal ArticleDOI
Quality Assessment of Fruits and Vegetables Based on Spatially Resolved Spectroscopy: A Review
TL;DR: In this article , the authors reviewed the applications of spatially resolved spectroscopy for measuring the quality attributes of fruits and vegetables in detail, including the principle of light transfer in biological tissues, diffusion approximation theory and methodologies.
Proceedings ArticleDOI
A sequential method for measuring the optical properties of two-layer media with spatially-resolved diffuse reflectance: simulation study
TL;DR: In this paper, a sequential method for estimating the optical properties of two-layer media with spatially-resolved diffuse reflectance was proposed and validated using Monte Carlo generated reflectance profiles.
Journal ArticleDOI
A sequential method for estimating the optical properties of two-layer agro-products from spatially-resolved diffuse reflectance: Simulation
Aichen Wang,Xinhua Wei +1 more
TL;DR: In this paper, a sequential method for estimating the optical properties of two-layer biological tissues with spatially-resolved diffuse reflectance was proposed and validated using Monte Carlo simulations, and the relationship between the penetration depth of detected photons and source-detector separation was first studied.
Journal ArticleDOI
Signal and measurement considerations for human translation of diffuse in vivo flow cytometry
TL;DR: The analysis of the impact of DiFC instrument geometry and wavelength on the detected DiFC signal and on the maximum depth of detection of a moving cell suggests that circulating cells and nanosensors could in principle be detectable in circulation in humans.
Posted ContentDOI
Signal and Measurement Considerations for Human Translation of Diffuse in vivo Flow Cytometry
TL;DR: In this paper , the authors used Monte Carlo simulations to compute Jacobian (sensitivity) matrices for a range of source-detector separations and tissue optical properties over the visible and near infrared (NIR) spectrum.
References
More filters
Journal ArticleDOI
In vitro fluorescence measurements and Monte Carlo simulation of laser irradiation propagation in porcine skin tissue.
TL;DR: In this paper, the authors performed Monte Carlo simulation of the light distribution in a five-layer model of human skin tissue, with a pulsed ultraviolet laser beam, and observed differences in the form and intensity of the fluorescence signal of the porcine skin, which can be attributed to different concentrations of the native fluorophores and the variable physical and biological conditions of the skin tissue.
Journal ArticleDOI
Infra-red Tomography of Port-wine-stain Blood Vessels in Human Skin
TL;DR: In this paper, the authors developed a tomographic reconstruction algorithm as a means to determine the depths and physical dimensions of discrete subsurface port-wine stain (PWS) blood vessels in human skin and the initial space-dependent temperature increase in PWS blood vessels immediately following pulsed laser exposure.
Proceedings ArticleDOI
Average depth of blood vessels in skin and lesions deduced by optical fiber spectroscopy
TL;DR: In this paper, the average depth of blood vessels in a cutaneous site, either normal or diseased, can be specified by a simple rapid noninvasive optical measurement, where the light reflected by tissue scattering is carried to a diode array spectrophotometer for spectral analysis.
Journal ArticleDOI
Imaging of an Absorbing Object Embedded in a Dense Scattering Medium by Diffusing Light Topography
Toshiaki Iwai,Gaku Kimura +1 more
TL;DR: In this paper, a simple imaging method for an absorbing object embedded in a dense scattering medium is proposed, which is based on the equivalence between a probability distribution function of the path-length and a backscattered intensity distribution integrated spatially in the boundary plane between the medium and the air.
Journal Article
Determination of optimal source-detector separation in measuring chromophores in layered tissue with diffuse reflectance
TL;DR: In this article, an optimal source-detector separation is derived from the mean penetration depth referring to monitoring the change of chromophores concentration of the sandwiched layer in a three-layer model with the method of Monte-Carlo simulation.
Related Papers (5)
Rapid and accurate estimation of blood saturation, melanin content, and epidermis thickness from spectral diffuse reflectance
Dmitry Yudovsky,Laurent Pilon +1 more