Journal ArticleDOI
Oxygen transfer effects in serine alkaline protease fermentation by Bacillus licheniformis: use of citric acid as the carbon source
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TLDR
The oxygen transfer coefficient increases with the agitation rate and the oxygen consumption rate increased almost linearly with the biomass concentration; however, substrate consumption was highest at HOT conditions.About:
This article is published in Enzyme and Microbial Technology.The article was published on 1998-11-15. It has received 107 citations till now. The article focuses on the topics: Citric acid & Oxygen.read more
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Journal ArticleDOI
Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes
TL;DR: The increase in casein concentration has resulted in increased viscosity of the fermentation broth with increase in time of bioreactor operation, which in turn resulted in decreased oxygen mass transfer coefficient with reduced oxygen transfer rates.
Journal ArticleDOI
Techniques for oxygen transfer measurement in bioreactors: a review
TL;DR: The present paper gives an overview of various measurement techniques andirlimitations and/ororsuitability for measurement of OTR/kLa in various kinds of bioreactors, especially small bioreActors.
Journal ArticleDOI
Fed‐batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co‐substrate sorbitol
TL;DR: Adding sorbitol batch‐wise to the medium provided the following advantages over growth on methanol alone: eliminating the long lag‐phase for the cells and reaching ‘high cell density production’ at t = 24 h of the process, and obtaining 1.4‐fold higher overall yield coefficients.
Journal ArticleDOI
Use of Biodiesel Byproduct Crude Glycerol as the Carbon Source for Fermentation Processes by Recombinant Pichia pastoris
TL;DR: A potential use for crude glycerol, without any purification, in the production of additional biodiesel products is demonstrated, having values approximately 1.4-fold higher than those attained with pure glycerols.
Journal ArticleDOI
Oxygen transfer effects on recombinant benzaldehyde lyase production
TL;DR: Oxygen transfer effects on benzaldehyde lyase (BAL) production by recombinant Escherichia coli carrying pUC18::bal gene were investigated on a defined medium with glucose and (NH 4 ) 2 HPO 4 as the sole carbon and nitrogen sources respectively, in 3.0m 3 bioreactor systems at six different conditions with the parameters, air inlet rate of Q O / V R = 0.5 vvm, and agitation rates of N = 250, 375, 500, 625, 750 ǫ min - 1 and at Q O/
References
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Bergey's Manual of Determinative Bacteriology.
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Rapid analysis of amino acids using pre-column derivatization.
TL;DR: The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
Book
Bacillus subtilis and other gram-positive bacteria : biochemistry, physiology, and molecular genetics
TL;DR: Gram-Positive Bacteria Metabolism and Regulation Cell Envelope Chromosome Structure chromosome Replication, Modification, and Repair Genetic Exchange and Genetic Engineering Transcription and Translation Machinery Post-Exponential Phase Phenomena Bacteriophages Production of Commercial Products.
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Extracellular enzyme synthesis in the genus Bacillus.
TL;DR: This article will attempt to review the recent literature concerned with the characterization and properties of the exoenzymes synthesized by the bacilli and the control and mechanisms of their synthesis.
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Mass transfer in gas-liquid slurry reactors
TL;DR: A critical review on the mass transfer characteristics of gas?liquid slurry reactors is presented in this article, where the authors discuss the influence of the presence of solid particles on the following mass transfer parameters: volumetric gas? liquid mass transfer coefficients (kLa, kGa), liquid-side mass transfer coefficient (kL and kS), and specific gas?slurry contact area (a).