Journal ArticleDOI
Partial purification and properties of thiamine pyrophosphokinase from pig brain.
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TLDR
Pig brain thiamine pyrophosphokinase was purified 260-fold over extracts of brain acetone powder and a preliminary study of the kinetics of the reaction reveals that the enzyme may function via a partial "ping-pong" mechanism.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1975-08-26. It has received 28 citations till now. The article focuses on the topics: Thiamine & Pyrithiamine.read more
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Journal ArticleDOI
The role of thiamine in nervous tissue.
TL;DR: It is suggested that despite the lack of hard evidence, it is likely that the vitamin possesses this alternate function of thiamine.
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Mechanism of Thiamine Transport in Neuroblastoma Cells. Inhibition of a High Affinity Carrier by Sodium Channel Activators and Dependence of Thiamine Uptake on Membrane Potential and Intracellular Atp
Lucien Bettendorff,Pierre Wins +1 more
TL;DR: Thiamine transport in mouse neuroblastoma cells is studied to suggest a secondary active transport of thiamine, the main driving force being Thiamine phosphorylation rather than the sodium gradient.
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Thiamine in excitable tissues: reflections on a non-cofactor role.
TL;DR: Abbreviations: DIDS, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, HPLC, high-performance liquid chromatography, KGDH, a-ketoglutarate dehydrogenase, PDH, pyruvate dehydrogen enzyme, TDP, thiamine diphosphate; TDPase,Thiamine Diphosphatase, TIP, Thiamine triph
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Thiamine, thiamine phosphates, and their metabolizing enzymes in human brain.
TL;DR: There was no differences in the total thiamine content between biopsied and autopsied human brain, except that in the latter, Thiamine triphosphate was undetectable.
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Nervous tissue thiamine metabolism in vivo. II. Thiamine and its phosphoesters dynamics in different brain regions and sciatic nerve of the rat.
TL;DR: The shortest turnover time of TPP was found in the cerebellum, while the sciatic nerve exhibited the fastest renewal of T and TMP, suggesting that its function might be associated to a rapid conversion into chemically different forms.
References
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A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
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The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations
TL;DR: A nomenclature to facilitate discussion of possible mechanisms for enzyme-catalyzed reactions with more than one substrate or product is proposed and examples are given to illustrate the types of information about reaction mechanisms that can be obtained.
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