Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells.
L.S. Wise,H. Green +1 more
TLDR
The adipose conversion may activate a hitherto silent gene for the stable enzyme, an important enzyme in triglyceride synthesis, if the two forms of glycerophosphate dehydrogenase are the products of independent genes.About:
This article is published in Journal of Biological Chemistry.The article was published on 1979-01-25 and is currently open access. It has received 503 citations till now. The article focuses on the topics: Enzyme inducer & Adipose tissue.read more
Citations
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Human Adipose Tissue Is a Source of Multipotent Stem Cells
Patricia A. Zuk,Min Zhu,Peter Ashjian,Daniel A. De Ugarte,Jerry I. Huang,Hiroshi Mizuno,Zeni Alfonso,John K. Fraser,Prosper Benhaim,Marc H. Hedrick +9 more
TL;DR: To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches and PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.
Journal ArticleDOI
Comparison of Multi-Lineage Cells from Human Adipose Tissue and Bone Marrow
Daniel A. De Ugarte,Kouki Morizono,Amir Elbarbary,Zeni Alfonso,Patricia A. Zuk,Min Zhu,Jason L. Dragoo,Peter Ashjian,Bert Thomas,Prosper Benhaim,Irvin S. Y. Chen,John K. Fraser,Marc H. Hedrick +12 more
TL;DR: This study is the first comparison of PLA cells and MSCs isolated from the same patient, and no significant differences were observed for yield of adherent stromal cells, growth kinetics, cell senescence, multi-lineage differentiation capacity, and gene transduction efficiency.
Journal ArticleDOI
Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O.
TL;DR: A fast and simple method to quantitate the extent of adipose conversion by staining the accumulated lipid with Oil red O and determining the amount of extracted dye at 510 nm is reported.
Journal ArticleDOI
Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes.
TL;DR: Preadipocytes have the potential for a maximal response to IGF-I, whereas the accumulation of more than 95% of adipocyte insulin receptors and the appearance of responsiveness to insulin are consequences of differentiation.
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Molecular cloning of mRNA from 3T3 adipocytes. Regulation of mRNA content for glycerophosphate dehydrogenase and other differentiation-dependent proteins during adipocyte development.
TL;DR: The time course of mRNA accumulation was different for each of these mRNAs, indicating they do not respond synchronously during differentiation, and a recombinant bacterial library containing cDNA prepared from mRNA of adipose 3T3 cells is constructed.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines
George J. Todaro,Howard Green +1 more
TL;DR: Disaggregated mouse embryo cells, grown in monolayers, underwent a progressive decline in growth rate upon successive transfer, the rapidity of the decline depending on the inoculation density, but nearly all cultures developed into established lines within 3 months of culture.
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Sublines of mouse 3T3 cells that accumulate lipid
Howard Green,Olaniyi Kehinde +1 more
TL;DR: Two clonal sublines are isolated from the established mouse fibroblast line 3T3 that accumulate large amounts of triglyceride fat when the cells are in the resting state and are reduced by lipolytic agents.
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Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells
Howard Green,Olanlyl Kehinde +1 more
TL;DR: Starting with a clone converting to adipose cells with a vary low frequency, it has been possible, by serial selection, to generate subclones which convert with a high frequency, due to spontaneous heritable changes affecting susceptibility to the adipose conversion.
Journal ArticleDOI
Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells.
TL;DR: This new procedure permitted an analysis of insulin binding activity and cellular responsiveness to insulin during adipocyte conversion in vitro and found that cells appeared to be adipocytes by morphological and biochemical criteria and the specific insulin binding was 6to lo-fold greater than in undifferentiated control cells.
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An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion
Howard Green,Olaniyi Kehinde +1 more