Journal ArticleDOI
Pax8: a marker for carcinoma of Müllerian origin in serous effusions.
Guo-Xia Tong,Kalpana Devaraj,Diane Hamele-Bena,Woojin M. Yu,Andrew T. Turk,Xiaowei Chen,Jason D. Wright,Ellen Greenebaum +7 more
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TLDR
Examination of PAX8 expression in the normal uterine corpus and cervix, malignant tumors arising from these sites, and malignant effusions suggests that PAX8 is an additional IHC marker for carcinomas of Müllerian origin, and recommends including PAX8 for evaluation of malignant serous effusions in women.Abstract:
PAX8 is a nuclear transcription factor with limited expression in normal and neoplastic tissues in a cell lineage-dependent manner. PAX8 has been detected in embryonic Mullerian ducts, human fallopian tubes, and ovarian carcinomas. However, little is known about its expression in other areas of the female genital tract. In this study, we used immunohistochemistry (IHC) to examine PAX8 expression in the normal uterine corpus and cervix, malignant tumors arising from these sites, and malignant effusions. We reported here that PAX8 was also detected in endometrial epithelial cells and endocervical glands, with a lower expression level in the latter, but not in the stromal cells of these areas. All endometrial carcinomas (N = 52) were positive for PAX8, whereas endocervical adenocarcinomas (N = 5) and uterine leiomyosarcomas (N = 3) were negative for PAX8. PAX8 was detected in 70% (22/31) and 68.8% (11/16) of metastatic carcinomas of the ovary and endometrium in serous effusions, respectively. No PAX8 was detected in carcinomas of nongynecologic origin or noncarcinomas (N = 71) in serous effusions except in one renal-cell carcinoma and one carcinoma of unknown primary in a woman. In addition, papillary serous carcinomas of the peritoneum (N = 10) were diffusely positive for PAX8, implying a Mullerian origin similar to malignant tumors in the female genital tract. Our findings suggest that PAX8 is an additional IHC marker for carcinomas of Mullerian origin hence we recommend including PAX8 for evaluation of malignant serous effusions in women, especially when tumors of Mullerian origin are in the differential diagnosis.read more
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Journal ArticleDOI
A comprehensive analysis of PAX8 expression in human epithelial tumors.
Anna Laury,Ruth Perets,Huiying Piao,Jeffrey F. Krane,Justine A. Barletta,Christopher A. French,Lucian R. Chirieac,Rosina T. Lis,Massimo Loda,Jason L. Hornick,Ronny Drapkin,Michelle S. Hirsch +11 more
TL;DR: Results show that PAX8 is a highly sensitive marker for thyroid, renal, Müllerian, and thymic tumors, and is an excellent marker for confirming primary tumor site.
Journal ArticleDOI
Tubal origin of ‘ovarian’ low-grade serous carcinoma
Jie Li,Nisreen Abushahin,Shujie Pang,Li Xiang,Li Xiang,Setsuko K. Chambers,Oluwole Fadare,Beihua Kong,Wenxin Zheng +8 more
TL;DR: In this paper, the origins of low-grade serous carcinomas (tubal versus ovarian) were investigated by comparatively evaluating the morphologic (secretory and ciliated cell distribution) and immunophenotypic (using antibodies to PAX8, tubulin, calretinin, and Ki67) attributes of its putative precursor lesions, the normal tubal epithelium and the overt malignancy.
Journal ArticleDOI
Value of PAX 8 immunostaining in tumor diagnosis: a review and update.
TL;DR: Analysis of published studies indicates that PAX2 sensitivity for epithelial renal neoplasms and epithelial tumors of the female genital tract is lower than that of PAX8, however, PAX2 does not appear to be expressed in epithelial tumor of the thyroid gland or thymus.
Journal ArticleDOI
Expanding the clinical phenotype of hereditary BAP1 cancer predisposition syndrome, reporting three new cases
Robert Pilarski,Colleen M. Cebulla,James B. Massengill,Karan Rai,Thereasa A. Rich,Louise C. Strong,Barbara Mcgillivray,Mary Jill Asrat,Frederick H. Davidorf,Mohamed H. Abdel-Rahman +9 more
TL;DR: The association between germline BAP1 mutation and predisposition to UM, mesothelioma, CM and RCC is confirmed, however, other cancers, such as cholangiocarcinoma and breast carcinoma may be part of the phenotype of this hereditary cancer predisposition syndrome.
Journal ArticleDOI
Application of immunohistochemistry in the diagnosis of epithelioid mesothelioma: a review and update.
TL;DR: A large number of immunohistochemical markers that can assist in the differential diagnosis of epithelioid mesotheliomas are currently available as mentioned in this paper, but their selection for inclusion in a diagnostic panel largely depends on the differential diagnoses, as well as on which ones work the best in a given laboratory.
References
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Female development in mammals is regulated by Wnt-4 signalling
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Cytokeratin 7 and Cytokeratin 20 Expression in Epithelial Neoplasms: A Survey of 435 Cases
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Pax8, a murine paired box gene expressed in the developing excretory system and thyroid gland.
Dimitrij Plachov,Kamal Chowdhury,Claudia Walther,Dominique Simon,Jean-Louis Guénet,Peter Gruss +5 more
TL;DR: The isolation of Pax8, a novel paired box containing clone from an 8.5 day p.c. mouse embryo cDNA library is described and the embryonic expression pattern is compared with that of Pax2, another recently described paired box gene expressed in the developing excretory system.
Journal ArticleDOI
The distal fallopian tube: a new model for pelvic serous carcinogenesis.
Christopher P. Crum,Ronny Drapkin,Alexander Miron,Tan A. Ince,Michael G. Muto,David Kindelberger,Yonghee Lee +6 more
TL;DR: The emerging data offer compelling evidence for a model of ‘fimbrial-ovarian’ serous neoplasia, and call attention to the distal fallopian tube as an important source for this disease, the study of which could clarify pathways to cancer in both organs and generate novel strategies for cancer prevention.
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Pulmonary carcinomas with pleomorphic, sarcomatoid, or sarcomatous elements: a clinicopathologic and immunohistochemical study of 75 cases.
Giulio Rossi,Alberto Cavazza,Nathalie Sturm,Mario Migaldi,Nicola Facciolongo,Lucia Longo,Antonio Maiorana,Elisabeth Brambilla +7 more
TL;DR: It is shown that cytokeratin 7 and TTF-1, but not surfactant protein-A, are useful immunohistochemical markers in this setting, and shows that this group of tumors has a worse prognosis than conventional non-small cell lung carcinoma at surgically curable stages I.