Journal ArticleDOI
Phospholipid asymmetry of goat sperm plasma membrane during epididymal maturation
TLDR
The data demonstrate asymmetry of phospholipids and their fatty acids in the sperm inner and outer plasma membranes and this lipid asymmetry is greatly altered during epididymal maturity of the male gametes.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1993-12-02. It has received 25 citations till now. The article focuses on the topics: Sperm plasma membrane & Sperm.read more
Citations
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Journal ArticleDOI
Dynamics of the mammalian sperm plasma membrane in the process of fertilization.
TL;DR: Dynamics in adhesive and fusion properties, molecular composition and architecture of the sperm plasma membrane, as well as membrane derived signalling are reviewed.
Journal ArticleDOI
Bicarbonate/CO2, an effector of capacitation, induces a rapid and reversible change in the lipid architecture of boar sperm plasma membranes
TL;DR: It is found that bicarbonate causes a rapid increase in the ability of live boar spermatozoa to bind merocyanine, apparently by perturbing enzymic control processes.
Journal ArticleDOI
Lipid heterogeneity and membrane fluidity in a highly polarized cell, the mammalian spermatozoon
TL;DR: This review summarizes current knowledge of how the membrane organization of the mammalian spermatozoon is determined by its complex mixture of lipids.
Journal ArticleDOI
Changes in lipids and membrane anisotropy in human spermatozoa during epididymal maturation.
Gerhard Haidl,C Opper +1 more
TL;DR: During epididymal maturation human spermatozoa integrate lipids, particularly PC, which is strongly associated with the induction of progressive motility, and a change in the pattern of fatty acids and a decrease in the cholesterol/phospholipid molar ratio cause a decreases in membrane anisotropy in cauda spermatozosa.
Journal ArticleDOI
Flow cytometric detection of transbilayer movement of fluorescent phospholipid analogues across the boar sperm plasma membrane: elimination of labeling artifacts.
Bart M. Gadella,Bart M. Gadella,N.G.A. Miller,Ben Colenbrander,L.M.G. Van Golde,R.A.P. Harrison +5 more
TL;DR: A method based on fluorescence resonance energy transfer was used to ensure that incorporation of the fluorescent phospholipids into the sperm proceeded via monomeric transfer and enables, for the first time, assessment of changes in lipid asymmetry under fertilizing conditions.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
Modern methods of plant analysis
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Development changes occurring in the lipids of ram epididymal spermatozoa plasma membrane.
John E. Parks,Roy H. Hammerstedt +1 more