Journal ArticleDOI
Photoexcited CRY2 Interacts with CIB1 to Regulate Transcription and Floral Initiation in Arabidopsis
TLDR
The identification and characterization of the Arabidopsis CIB1 (cryptochrome-interacting basic-helix-loop-Helix) protein is reported, and it is proposed that the blue light–dependent interaction of cryptochrome(s) with C IB1 and CIB 1-related proteins represents an early photoreceptor signaling mechanism in plants.Abstract:
Cryptochromes (CRY) are photolyase-like blue-light receptors that mediate light responses in plants and animals. How plant cryptochromes act in response to blue light is not well understood. We report here the identification and characterization of the Arabidopsis CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein. CIB1 interacts with CRY2 (cryptochrome 2) in a blue light-specific manner in yeast and Arabidopsis cells, and it acts together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. CIB1 binds to G box (CACGTG) in vitro with a higher affinity than its interaction with other E-box elements (CANNTG). However, CIB1 stimulates FT messenger RNA expression, and it interacts with chromatin DNA of the FT gene that possesses various E-box elements except G box. We propose that the blue light-dependent interaction of cryptochrome(s) with CIB1 and CIB1-related proteins represents an early photoreceptor signaling mechanism in plants.read more
Citations
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Journal ArticleDOI
The genetic basis of flowering responses to seasonal cues
Fernando Andrés,George Coupland +1 more
TL;DR: Progress is reported in defining the diverse genetic mechanisms that enable plants to recognize winter, spring and autumn to initiate flower development.
Journal ArticleDOI
Perception of UV-B by the arabidopsis UVR8 protein
Luca Rizzini,Jean Jacques Favory,Catherine Cloix,Davide Faggionato,Andrew O'Hara,Eirini Kaiserli,Ralf Baumeister,Eberhard Schäfer,Ferenc Nagy,Ferenc Nagy,Gareth I. Jenkins,Roman Ulm,Roman Ulm +12 more
TL;DR: It is shown that dimers of the UVR8 protein perceive UV-B, probably by a tryptophan-based mechanism, which mediatesUV-B photomorphogenic responses securing plant acclimation and thus promotes survival in sunlight.
Journal ArticleDOI
Rapid blue-light-mediated induction of protein interactions in living cells
Matthew J. Kennedy,Robert M. Hughes,Leslie A. Peteya,Joel W. Schwartz,Michael D. Ehlers,Michael D. Ehlers,Chandra L. Tucker +6 more
TL;DR: Genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution are described.
Journal ArticleDOI
Optical control of mammalian endogenous transcription and epigenetic states
Silvana Konermann,Silvana Konermann,Mark D. Brigham,Mark D. Brigham,Mark D. Brigham,Alexandro E. Trevino,Alexandro E. Trevino,Patrick D. Hsu,Patrick D. Hsu,Patrick D. Hsu,Matthias Heidenreich,Matthias Heidenreich,Le Cong,Le Cong,Le Cong,Randall Jeffrey Platt,Randall Jeffrey Platt,David A. Scott,David A. Scott,George M. Church,George M. Church,Feng Zhang,Feng Zhang +22 more
TL;DR: The development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana, establishes a novel mode of optogenetics control of endogenous cellular processes and enables direct testing of the causal roles of genetic and epigenetic regulation in normal biological processes and disease states.
Journal ArticleDOI
The Cryptochromes: Blue Light Photoreceptors in Plants and Animals
Inês Chaves,Richard Pokorny,Martin Byrdin,Nathalie Hoang,Thorsten Ritz,Klaus Brettel,Lars-Oliver Essen,Gijsbertus T. J. van der Horst,Alfred Batschauer,Margaret Ahmad +9 more
TL;DR: The structural, photochemical, and molecular properties of cry-DASH, plant, and animal cryptochromes are reviewed in relation to biological signaling mechanisms and common features that may contribute to better understanding the function of cryptochromaes in diverse systems including in man are uncovered.
References
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Roger P. Hellens,Andrew C. Allan,Ellen N. Friel,Karen Bolitho,Karryn Grafton,Matthew D. Templeton,Sakuntala Karunairetnam,Andrew P. Gleave,William A. Laing +8 more
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Journal ArticleDOI
Photoreceptor regulation of CONSTANS protein in photoperiodic flowering
Frederico. Valverde,Aidyn Mouradov,Wim J. J. Soppe,Dean Ravenscroft,Alon Samach,George Coupland +5 more
TL;DR: It is shown that light stabilizes nuclear CO protein in the evening, whereas in the morning or in darkness the protein is degraded by the proteasome.
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HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor
TL;DR: The hy4 mutant5 is one of several mutants that are selectively insensitive to blue light during the blue-light-dependent inhibition of hypocotyl elongation response, which suggests that they lack an essential component of the cryptochrome-associated light-sensing pathway.
Journal ArticleDOI
The Arabidopsis basic/helix-loop-helix transcription factor family.
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