Preparation and characterization of a hormone-responsive renal plasma membrane fraction.
TLDR
A renal plasma membrane fraction has been prepared by homogenization and differential centrifugation in an isotonic sucrose solution in 10 mm Tris, pH 7.5, and 1 mm ethylenediaminetetraacetate that showed a striking decrease in specific activity of marker enzymes for endoplasmic reticulum and brush border, but a 2.7-fold increase in thespecific activity of Na-K-ATPase.About:
This article is published in Journal of Biological Chemistry.The article was published on 1972-11-10 and is currently open access. It has received 153 citations till now. The article focuses on the topics: Cyclase activity & Calcitonin.read more
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Journal ArticleDOI
HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney.
Michael J. Ryan,Gretchen Johnson,J Kirk,Sally M. Fuerstenberg,Richard A. Zager,Beverly Torok-Storb +5 more
TL;DR: An immortalized adult human PTC line has been established by transduction with HPV 16 E6/E7 genes and it appears to be well-differentiated on the basis of its histochemical, immune cytochemical, and functional characteristics, and it can reproduce experimental results obtained with freshly isolated PTCs.
Book ChapterDOI
Membrane receptors and hormone action.
TL;DR: This chapter describes the relation between biological activity and binding, physicochemical detection and assay of receptors, solubilization of membrane receptors, examples of membrane-localized receptors, affinity techniques, polyvalent hormone derivatives, and receptor studies, and theoretical mechanisms of hormone–receptor binding and action.
Journal ArticleDOI
Peripheral benzodiazepine binding sites: effect of PK 11195, 1-(2-chlorophenyl)-N-methyl-(1-methylpropyl)-3 isoquinolinecarboxamide. II. In vivo studies.
G. Le Fur,M.L. Perrier,Nadine Vaucher,F. Imbault,A. Flamier,J. Benavides,Andre Uzan,Christian Renault,Marie-Christine Dubroeucq,C. Gueremy +9 more
TL;DR: In this article, a new compound, chemically unrelated to benzodiazepines, which is a potent inhibitor of [ 3 H] R05-4864 in vitro is also very effective after I.P. injection and oral administration.
Journal ArticleDOI
Peripheral benzodiazepine binding sites: Effect of PK 11195, 1-(2-chlorophenyl)-n-methyl-n-(1-methylpropyl)-3-isoquinolinecarboxamide
G. Le Fur,M.L. Perrier,Nadine Vaucher,F. Imbault,A. Flamier,J. Benavides,Andre Uzan,Christian Renault,Marie-Christine Dubroeucq,C. Gueremy +9 more
Journal ArticleDOI
Interaction of calcitonin and calcitonin gene-related peptide at receptor sites in target tissues.
David Goltzman,Jane Mitchell +1 more
TL;DR: The relative abilities of C GRP and mammalian CT to inhibit CT binding suggest that CGRP could serve as an endogenous ligand for CT receptors in the central nervous system.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
Journal ArticleDOI
A modified method for the isolation of the plasma membrane from rat liver
TL;DR: A modified method for the isolation of the plasma membrane from rat liver has been developed and the concentration of Ca2+ and the degree of the homogenizing medium are critical factors in obtaining membranes in high yield and with high activity of the characteristic plasma membrane enzymes.
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