Journal ArticleDOI
Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays.
Mark L. Collins,C Zayati,Jill Detmer,B. Daly,Janice A. Kolberg,Tai-An Cha,Irvine Bruce,J. Tucker,Mickey S. Urdea +8 more
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TLDR
Standard Hepatitis C virus (HCV) RNAs showed that size has no detectable effect on quantitation in the branched DNA hybridization assay, and standard HCV RNA transcripts were prepared from clones of HCV subtypes 1b and 3a to study the effects of target sequence diversity and probe design on quantification by hybridization.About:
This article is published in Analytical Biochemistry.The article was published on 1995-03-01. It has received 125 citations till now. The article focuses on the topics: Hybridization probe & RNA.read more
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A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics.
Weihong Liu,David A. Saint +1 more
TL;DR: Based on the simulation of kinetic process of real-time PCR, a new method for quantitation and normalization of gene transcripts is developed that provides a simple and accurate approach to quantifying gene expression level with the advantages that neither construction of standard curve nor validation experiments are needed.
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Progesterone implants enhance SIV vaginal transmission and early virus load
Preston A. Marx,A I Spira,A I Spira,A Gettie,Peter J. Dailey,Ronald S. Veazey,Andrew A. Lackner,C J Mahoney,Christopher J. Miller,L E Claypool,David D. Ho,Nancy J. Alexander +11 more
TL;DR: This study shows that SIV genital infection and disease course are enhanced by subcutaneous implants containing progesterone when compared with the rate of vaginal transmission in the follicular phase.
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Validation of a quantitative method for real time PCR kinetics.
Weihong Liu,David A. Saint +1 more
TL;DR: A new quantitative method is proposed which represents a simple but accurate quantitative method which is capable of measuring cycle-by-cycle PCR amplification efficiencies and demonstrates that these change dynamically.
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A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml
Mark L. Collins,Irvine Bruce,Diana Tyner,Eric Fine,Crystle L. K. Zayati,Chu-An Chang,Thomas Horn,David Ahle,Jill Detmer,Lu-Ping Shen,Janice A. Kolberg,Steve Bushnell,Mickey S. Urdea,David D. Ho +13 more
TL;DR: The branched DNA hybridization assay has been improved by the inclusion of the novel nucleotides, isoC and isoG, in the amplification sequences to prevent non-specific hybridization and permits increased signal amplification.
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Developments in quantitative PCR
TL;DR: The role of reference materials and calibrators and the different strategies adopted for nucleic acid quantification are reviewed and a recent promising technology for quantitative PCR in which the use of fluorogenic probes and dedicated instrumentation allows the development of homogeneous methods is dedicated.