Preparation of high titer lambda phage lysates
Thomas A. Patterson,Michael Dean +1 more
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This article is published in Nucleic Acids Research.The article was published on 1987-08-11 and is currently open access. It has received 51 citations till now. The article focuses on the topics: Cosmid & Lambda phage.read more
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Identification of the cystic fibrosis gene: Chromosome walking and jumping
Johanna M. Rommens,Michael C. Iannuzzi,Batsheva Kerem,Mitchell L. Drumm,Georg Melmer,Michael Dean,Richard Rozmahel,Jeffery L. Cole,Dara Kennedy,Noriko Hidaka,Martha Zsiga,Manuel Buchwald,John R. Riordan,Lap-Chee Tsui,Francis S. Collins +14 more
TL;DR: Several transcribed sequences and conserved segments were identified in this cloned region and one corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
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Identification of a chromosome 18q gene that is altered in colorectal cancers
Eric R. Fearon,Kathleen R. Cho,Janice M. Nigro,Scott E. Kern,Jonathan W. Simons,J. Michael Ruppert,Stanley R. Hamilton,Antonette C. Preisinger,Giles Thomas,Kenneth W. Kinzler,Bert Vogelstein +10 more
TL;DR: A contiguous stretch of DNA comprising 370 kilobase pairs has now been cloned from a region of chromosome 18q suspected to reside near the DCC gene, which may play a role in the pathogenesis of human colorectal neoplasia, perhaps through alteration of the normal cell-cell interactions controlling growth.
Journal ArticleDOI
Molecular cloning of a human fucosyltransferase gene that determines expression of the Lewis x and VIM-2 epitopes but not ELAM-1-dependent cell adhesion.
John B. Lowe,Jolanta F. Kukowska-Latallo,Rajan P. Nair,Robert D. Larsen,Rory M. Marks,B.A. Macher,Robert Kelly,L. K. Ernst +7 more
TL;DR: It is demonstrated that substantial structural similarities can exist between mammalian glycosyltransferases with closely related enzymatic properties, thus facilitating isolation of their cognate genes by cross-hybridization methods.
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Cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene and cDNA from the plant pathogenic fungus Glomerella cingulata.
TL;DR: The glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) has been identified from a genomic DNA library prepared from the plant pathogenic fungus Glomerella cingulata and the general structure of the 5' flanking region shows similarities to gpdA from Aspergillus nidulans.
Journal ArticleDOI
Family of the major cold‐shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y‐box binding proteins
TL;DR: Indicate that E. coli has a family of the cspA gene, some of which are induced by cold shock, and two independent clones from the Kohara miniset phage collection, which hybridized with a DNA fragment containing cSpA are found.