Journal ArticleDOI
Probing oxidative stress in single erythrocytes with Raman Tweezers
TLDR
There is significant enhancement in Raman peak intensities corresponding to SS and C-S stretching modes that are induced by oxidative stress, consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells.Abstract:
Raman Tweezers have been successfully applied to characterize chemically-induced oxidative stress on optically-trapped live, single erythrocytes. There is significant enhancement in Raman peak intensities corresponding to SS and C-S stretching modes that are induced by oxidative stress. This is consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells. Enhancement in glutathione level as a protective response against oxidative stress has been observed. Principal component analysis of the data yields good discrimination between spectra of normal and stress-induced red blood cells.read more
Citations
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Journal ArticleDOI
Surface-enhanced Raman scattering of whole human blood, blood plasma, and red blood cells: cellular processes and bioanalytical sensing.
TL;DR: SERS spectra of whole human blood, blood plasma, and red blood cells on Au nanoparticle SiO(2) substrates excited at 785 nm have been observed and can have significant impact in the area of clinical diagnostics, blood supply management, and forensics.
Journal ArticleDOI
Raman Spectroscopy of Blood and Blood Components
TL;DR: The literature in the field is reviewed, the published Raman spectroscopy studies of erythrocytes, leucocytes, platelets, plasma, and whole blood are collated, and general conclusions on the state of the field are drawn.
Journal ArticleDOI
Real-time monitoring of glutathione-triggered thiopurine anticancer drug release in live cells investigated by surface-enhanced Raman scattering.
Kwang-Su Ock,Won Il Jeon,Erdene-Ochir Ganbold,Mira Kim,Jin-Ho Park,Ji Hye Seo,Keunchang Cho,Sang-Woo Joo,So Yeong Lee +8 more
TL;DR: In vitro and in vivo glutathione (GSH)-induced intracellular thiopurine anticancer drug release on gold nanoparticle (Au NP) surfaces is investigated by means of label-free confocal Raman spectroscopy and demonstrates that the time-lapse Ramans spectroscopic tools are useful for monitoring of the controlled release of thioporine drug molecules in vitro andIn vivo.
Journal ArticleDOI
Recent advances in laser tweezers Raman spectroscopy (LTRS) for label-free analysis of single cells.
TL;DR: An overview of the LTRS method is provided, with an emphasis on highlighting recent advances over the past several years in the development of the technology and several new biological and biomedical applications that have been demonstrated.
Journal ArticleDOI
Optical Tweezers in Studies of Red Blood Cells.
TL;DR: The background of OTs, their state-of-the-art applications in exploring single-cell level characteristics and bio-rheological properties of mature red blood cells (RBCs) as well as the OTs-assisted studies on erythropoiesis are summarized and presented.
References
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TL;DR: In conclusion, acetaminophen hepatotoxicity far exceeds other causes of acute liver failure in the United States, and education of patients, physicians, and pharmacies to limit high‐risk use settings is recommended.
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Acetaminophen-induced hepatotoxicity
TL;DR: The recent finding that NADPH oxidase knockout mice were equally sensitive to acetaminophen and had equal nitration of tyrosine suggests that the superoxide is not from the activation of Kupffer cells.
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Assignment of Protoheme Resonance Raman Spectrum by Heme Labeling in Myoglobin
TL;DR: In this paper, the Resonance Raman (RR) spectra are reported for myoglobin reconstituted with seven heme isotopomers which are labeled with 15N and meso-D4 in the porphyrin skeleton or at the propionate substituents.
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Personal PM2.5 exposure and markers of oxidative stress in blood.
Mettte Sørensen,Bahram Daneshvar,Max Hansen,Lars O. Dragsted,Ole Hertel,Lisbeth E. Knudsen,Steffen Loft +6 more
TL;DR: Personal exposure appears more closely related to these biomarkers potentially related to cardiovascular disease than is ambient PM(2.5) background concentrations, and exposure to particles in moderate concentrations can induce oxidative stress and increase RBCs in peripheral blood.
Journal ArticleDOI
Resonance Raman spectroscopy of red blood cells using near-infrared laser excitation.
TL;DR: New insights are provided into the vibrational dynamics, electronic structure and resonant enhancement of heme moieties within functional erythrocytes at near-IR excitation wavelengths and non-totally symmetric B1g modes in oxygenated cells.