Open AccessJournal Article
Protein quantification with coomassie bulliant blue microplate-colormetric
Reads0
Chats0
TLDR
MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.Abstract:
AIM To develop a rapid and sensitive method of protein quantification assay. METHODS Protein content of bovine serum albumin (BSA) and mice liver homogenate was determinated by microplate colormetric (MPC) in which coomassie brilliant blue (CBB) is used as color reagent. RESULTS Detecting limit of MPC was lower (0.63 μg) than that of macro colormetric (MC). MPC had advantage of rapidity and lesser volume of sample (a few microliter). CONCLUSION MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.read more
Citations
More filters
Journal ArticleDOI
Antitumor activity of polysaccharides isolated from Patrinia heterophylla.
TL;DR: PHB-P1 could inhibit tumor growth and induce tumor cell apoptosis, and might up-regulate the expression of p53 and Bax, and significantly inhibited theexpression of Bcl-2 in tumor tissues.
Journal ArticleDOI
Dose-Dependent Effect of ZnO Quantum Dots for Lettuce Growth.
TL;DR: ZnO QDs at this level promoted Ca, Mg, Fe, Mn, Zn, and B absorption and accumulation; increased soluble sugar content; and improved the lettuce biomass and nutritional quality.
Proceedings ArticleDOI
Isolation, purification and structure characteristics of polysaccharide from Portulaca oleracea L.
TL;DR: In this paper, the technology of separating and purificating polysaccharides from Portulaca oleracea was studied by using high-performance gel-permeation chromatography (HPGPC) and the gas chromatography, respectively.
Proceedings ArticleDOI
Isolation, purification and structure characteristics of polysaccharide from Scutellaria barbata
TL;DR: In this paper, water-soluble polysaccharides named SBP-11, SBP12,SBP-13 and SBP14 were obtained from Scutellaria barbata by defatting, boiling water extraction, deproteinating, decoloration and DEAE-52 chromatography purificaton.
Journal Article
[Expression and purification of fusion protein CTP-SOD in Pichia pastoris and antioxidant capacity analysis].
TL;DR: CTP-SOD fusion gene was amplified from human cDNA by PCR, and the active recombinant protein was successfully expressed in Pichia pastoris and showed a significantly improved survival against the pyrogallol-induced oxidative stress, suggesting CTP-S OD could cross the cell membrane more efficiently and protect cells from oxidative stress.
Related Papers (5)
A modified Coomassie Brilliant Blue staining method at nanogram sensitivity compatible with proteomic analysis.
Xuchu Wang,Xiaofang Li,Yinxin Li +2 more
Evaluation of an improved Coomassie dye binding method for urinary protein assay
Michel Macart,Lucile Gerbaut +1 more