Proteome Analysis of Walnut Bacterial Blight Disease
Cíntia H. D. Sagawa,Renata de Almeida Barbosa Assis,Renata de Almeida Barbosa Assis,Paulo A. Zaini,Phillip A. Wilmarth,Brett S. Phinney,Leandro Marcio Moreira,Abhaya M. Dandekar +7 more
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TLDR
Walnut hull tissues inoculated with Xaj were compared to mock-inoculated tissues, and 3972 proteins were identified, suggesting intense degradation processes, oxidative stress, and general arrest of the biosynthetic metabolism in infected nuts.Abstract:
The interaction between the plant host, walnut (Juglans regia; Jr), and a deadly pathogen (Xanthomonas arboricola pv. juglandis 417; Xaj) can lead to walnut bacterial blight (WB), which depletes walnut productivity by degrading the nut quality. Here, we dissect this pathosystem using tandem mass tag quantitative proteomics. Walnut hull tissues inoculated with Xaj were compared to mock-inoculated tissues, and 3972 proteins were identified, of which 3296 are from Jr and 676 from Xaj. Proteins with differential abundance include oxidoreductases, proteases, and enzymes involved in energy metabolism and amino acid interconversion pathways. Defense responses and plant hormone biosynthesis were also increased. Xaj proteins detected in infected tissues demonstrate its ability to adapt to the host microenvironment, limiting iron availability, coping with copper toxicity, and maintaining energy and intermediary metabolism. Secreted proteases and extracellular secretion apparatus such as type IV pilus for twitching motility and type III secretion effectors indicate putative factors recognized by the host. Taken together, these results suggest intense degradation processes, oxidative stress, and general arrest of the biosynthetic metabolism in infected nuts. Our results provide insights into molecular mechanisms and highlight potential molecular tools for early detection and disease control strategies.read more
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A comparative genomic analysis of Xanthomonas arboricola pv. juglandis strains reveal hallmarks of mobile genetic elements in the adaptation and accelerated evolution of virulence.
Renata de Almeida Barbosa Assis,Alessandro M. Varani,Cíntia H. D. Sagawa,José S. L. Patané,João C. Setubal,Guillermo Uceda-Campos,Aline Maria Da Silva,Paulo A. Zaini,Nalvo F. Almeida,Leandro Marcio Moreira,Abhaya M. Dandekar +10 more
TL;DR: In this paper, the authors examined the genetic repertoire associated with adaptation and virulence evolution in Xanthomonas arboricola pv. juglandis (Xaj) and revealed the possible acquisition and propagation of virulence factors via insertion sequences (IS).
Journal ArticleDOI
Comparative Proteomic Analysis of Walnut (Juglans regia L.) Pellicle Tissues Reveals the Regulation of Nut Quality Attributes.
Paulo A. Zaini,Noah G Feinberg,Filipa Grilo,Houston J Saxe,Michelle Salemi,Brett S. Phinney,Carlos H. Crisosto,Abhaya M. Dandekar +7 more
TL;DR: A deep comparative proteome analysis of kernel pellicle tissue from these two valued genotypes at three harvest maturities provides clues on antioxidant, secondary, and hormonal metabolism that could be involved in the loss of quality in the pellicles during processing for commercialization.
Journal ArticleDOI
De Novo Arginine Synthesis Is Required for Full Virulence of <i>Xanthomonas arboricola</i> pv. <i>juglandis</i> During Walnut Bacterial Blight Disease
TL;DR: In this paper , the effects of bacterial arginine and nitrogen metabolism on the plant response during Walnut blight (WB) by proteomic analysis of the mutant strain Xaj argG-.
Journal ArticleDOI
A Secreted Chorismate Mutase from Xanthomonas arboricola pv. juglandis Attenuates Virulence and Walnut Blight Symptoms.
Renata de Almeida Barbosa Assis,Cíntia H. D. Sagawa,Paulo A. Zaini,Houston J Saxe,Phillip A. Wilmarth,Brett S. Phinney,Michelle Salemi,Leandro Marcio Moreira,Abhaya M. Dandekar +8 more
TL;DR: In this paper, the secreted form of chorismate mutase (CM), a key enzyme of the shikimate pathway regulating plant immunity, is highly conserved between plant-associated beta and gamma proteobacteria including phytopathogens belonging to the Xanthomonadaceae family.
Journal ArticleDOI
Effects of Phenolic Compounds on Walnut Bacterial Blight in the Green Husk of Hungarian-Bred Cultivars
TL;DR: In this paper , the authors investigated the relationship between the presence and concentration of phenolic compounds and the tolerance/resistance of the observed cultivars to walnut bacterial blight and found that Hungarian-bred local cultivars contained phenolic compound in higher concentrations than the domesticated ones.
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