Journal ArticleDOI
Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins.
Micky D. Tortorella,Timothy Burn,Michael A. Pratta,Ilgar Abbaszade,Jeannine M. Hollis,Rui-Qin Liu,Stuart A. Rosenfeld,Robert A. Copeland,Carl P. Decicco,Richard Wynn,A. Rockwell,F. Yang,J. L. Duke,Kimberly A. Solomon,Henry J. George,Robert C. Bruckner,Hideaki Nagase,Yoshifumi Itoh,Dawn Ellis,Harold D. Ross,Barbara H. Wiswall,Kathleen Murphy,M.C. Hillman,Gregory Hollis,Robert C. Newton,Ronald L. Magolda,James M. Trzaskos,E. C. Arner +27 more
TLDR
The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.Abstract:
We purified, cloned, and expressed aggrecanase, a protease that is thought to be responsible for the degradation of cartilage aggrecan in arthritic diseases. Aggrecanase-1 [a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4)] is a member of the ADAMTS protein family that cleaves aggrecan at the glutamic acid-373-alanine-374 bond. The identification of this protease provides a specific target for the development of therapeutics to prevent cartilage degradation in arthritis.read more
Citations
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Journal ArticleDOI
How Matrix Metalloproteinases Regulate Cell Behavior
Mark D. Sternlicht,Zena Werb +1 more
TL;DR: Recent advances shed light on how the structure and function of the MMPs are related and on how their transcription, secretion, activation, inhibition, localization, and clearance are controlled.
Journal ArticleDOI
Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura
Gallia Levy,William C. Nichols,Eric C.-Y. Lian,Tatiana Foroud,Jeanette N. McClintick,Beth McGee,Angela Y. Yang,David R. Siemieniak,Kenneth R. Stark,Ralph A. Gruppo,Ravindra Sarode,Susan B. Shurin,Visalam Chandrasekaran,Sally P. Stabler,Hernan Sabio,Eric E. Bouhassira,Jefferson D. Upshaw,David Ginsburg,Han-Mou Tsai +18 more
TL;DR: In this article, the ADAMTS family of zinc metalloproteinase genes (ADAMTS13) was identified as the molecular mechanism responsible for TTP, and it was shown that the deficiency of ADADTS13 is the molecular mechanisms responsible for the development of TTP.
Journal ArticleDOI
Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis
S.S. Glasson,Roger Askew,Barbara Sheppard,Brenda A. Carito,Tracey Blanchet,Hak-Ling Ma,Carl R. Flannery,Peluso Diane,Kim Kanki,Zhiyong Yang,Manas K. Majumdar,Elisabeth A. Morris +11 more
TL;DR: It is demonstrated that ADAMTS5 is the primary ‘aggrecanase’ responsible for aggrecan degradation in a murine model of osteoarthritis, and rational strategies for therapeutic intervention in osteOarthritis are suggested.
Journal ArticleDOI
Matrix metalloproteinases: role in arthritis.
TL;DR: Increasing knowledge of the crystal structures of these enzymes and of the signal transduction pathways and molecular mechanisms that control MMP gene expression may provide new opportunities for the development of therapeutics to prevent the joint destruction seen in arthritis.
Journal ArticleDOI
Extracellular matrix and cell signalling: the dynamic cooperation of integrin, proteoglycan and growth factor receptor
TL;DR: This review focuses on the current understanding of integrins and heparan sulphate proteoglycans as the essential cellular machineries that sense, integrate and respond to the physical and chemical environmental information either by directly connecting with the local adhesion sites or by regulating global cellular processes through growth factor receptor signalling pathways, leading to the integration of both external and internal signals in space and time.
References
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Journal ArticleDOI
The cysteine switch: a principle of regulation of metalloproteinase activity with potential applicability to the entire matrix metalloproteinase gene family.
H E Van Wart,H Birkedal-Hansen +1 more
TL;DR: Observations support the view that the cysteine-switch mechanism is applicable to all members of this gene family and offers the opportunity for multiple modes of physiological activation of these important enzymes.
Journal ArticleDOI
The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression.
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Journal ArticleDOI
Prediction of the Coding Sequences of Unidentified Human Genes. X. The Complete Sequences of 100 New cDNA Clones from Brain Which Can Code for Large Proteins in vitro
Ken Ichi Ishikawa,Takahiro Nagase,Mikita Suyama,Nobuyuki Miyajima,Ayako Tanaka,Hirokazu Kotani,Nobuo Nomura,Osamu Ohara +7 more
TL;DR: The entire sequences of 100 cDNA clones which were screened on the basis of the potentiality of coding for large proteins in vitro were determined and the expression profiles in a variety of tissues and chromosomal locations of the sequenced clones have been determined.
Journal ArticleDOI
Mammalian subtilisins: The long-sought dibasic processing endoproteases
TL;DR: After many years of searching for the enzymes responsible for cleavage of mammalian proproteins and prohormones at sites comprised of pairs of basic amino acid residues (“dibasic sites”), the solution to this enigma of cell biology appears to be in sight.
Journal ArticleDOI
Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene.
TL;DR: The TSP homologous domain containing the TSP type I motif of ADAMTS-1 is functional for binding to heparin, suggesting that ADAM-TS- 1 may be a gene whose expression is associated with various inflammatory processes as well as development of cancer cachexia.
Related Papers (5)
Cloning and characterization of ADAMTS11, an aggrecanase from the ADAMTS family.
Ilgar Abbaszade,Rui-Qin Liu,Fude Yang,Stuart A. Rosenfeld,O. Harold Ross,John Link,Dawn Ellis,Micky D. Tortorella,Michael A. Pratta,Jeannine M. Hollis,Richard Wynn,J. L. Duke,Henry J. George,M.C. Hillman,Kathleen Murphy,Barbara H. Wiswall,Robert A. Copeland,Carl P. Decicco,Robert C. Bruckner,Hideaki Nagase,Yoshifumi Itoh,Robert C. Newton,Ronald L. Magolda,James M. Trzaskos,Gregory Hollis,Elizabeth C. Arner,Timothy Burn +26 more