Journal ArticleDOI
Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography
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TLDR
A new procedure is described for the purification of an anti-carcinoembryonic antigen (CEA) single chain Fv, referred to as MFE-23, from bacterial supernatant using a simple insertion of a hexa-histidine tail fused at the C-terminus, which proved to be superior to standard CEA antigen affinity chromatography.About:
This article is published in Journal of Immunological Methods.The article was published on 1995-01-01. It has received 134 citations till now. The article focuses on the topics: Affinity chromatography & Iminodiacetic acid.read more
Citations
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Journal ArticleDOI
Perspectives of immobilized-metal affinity chromatography.
TL;DR: Advantages of the method and new prospects are described as well as the problems and concerns that appear when the method is to be used for production of pharmaceutical-grade proteins.
Journal ArticleDOI
Design and application of diabodies, triabodies and tetrabodies for cancer targeting
Aneta Todorovska,R C Roovers,Olan Dolezal,Alexander A. Kortt,Hennie R. Hoogenboom,Peter J. Hudson +5 more
TL;DR: This review describes the design and expression of diabodies, triabodies and tetrabodies using examples of scFv molecules that target viruses and cancer and highlights a number of cancer-targeting scFV multimers that have recently successfully undergone pre-clinical trials for in vivo stability and efficacy.
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Targeting by affinity-matured recombinant antibody fragments of an angiogenesis associated fibronectin isoform.
Dario Neri,Barbara Carnemolla,Ahuva Nissim,Alessandra Leprini,Germano Querzé,Enrica Balza,Alessandro Pini,Lorenzo Tarli,Cornelia Halin,Paolo Neri,Luciano Zardi,Greg Winter +11 more
TL;DR: The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels and could provide a target for diagnostic imaging and therapy of cancer.
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High avidity scFv multimers; diabodies and triabodies
TL;DR: This review describes how careful choice of linker length between V-domains creates new types of FV modules with size, flexibility and valency suited to in vivo imaging and therapy and the design of multi-specific Fv modules suited to cross-linking target antigens for cell-recruitment, viral delivery and immunodiagnostics.
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Progress in protein and antibody microarray technology.
TL;DR: This review aims to introduce these new technologies and highlights their current prospects and limitations for the screening of complex protein samples.
References
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The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
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Metal chelate affinity chromatography, a new approach to protein fractionation
TL;DR: A highly flexible method based on affinities which can be used in a more selective fashion by modern chromatographic techniques is described here.
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New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.
TL;DR: A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography and has been found to chelate Cu2+ and Ni2+ strongly and to be superior to the known iminodiacetic acid Adsorbent.
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Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent
TL;DR: A general purification method for recombinant proteins based upon the selective interaction between a poly-histidine peptide, which is fused to the protein of interest, and a novel metal chelate adsorbent is described.
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Selection of phage antibodies by binding affinity. Mimicking affinity maturation.
TL;DR: To mimic the affinity maturation process of the immune system, random mutations into the antibody genes in vitro using an error-prone polymerase are introduced, and a mutant with a fourfold improved affinity to the hapten 4-hydroxy-5-iodo-3-nitrophenacetyl-(NIP)-caproic acid is isolated.