Journal ArticleDOI
Rapid chloroplast segregation and recombination of mitochondrial DNA in Brassica cybrids
Alison Morgan,Pal Maliga +1 more
TLDR
Physical mapping revealed recombination in a region which is not normally involved in the formation of subgenomic mtDNA circles in Brassica cybrids, and the role of treatments used to facilitate the recovery of cy hybrids, and of organelle compatibility in hybrid cytoplasm formation is discussed.Abstract:
Brassica cybrids were obtained after fusing protoplasts of fertile and cytoplasmic male sterile (CMS) B. napus lines carrying the original b. napus, and the Ogura Raphanus sativus cytoplasms, respectively. Iodoacetate treatment of the fertile line and X-irradiation of the CMS line prevented colony formation from the parental protoplasts. Colony formation, however, was obtained after protoplast fusion. Hybrid cytoplasm formation was studied in 0.5 g to 5.0 g calli grown from a fused protoplast after an estimated 19 to 22 cell divisions. Chloroplasts and mitochondria were identified in the calli by hybridizing appropriate DNA probes to total cellular DNA. Out of the 42 clones studied 37 were confirmed as cybrids. Chloroplast segregation was complete at the time of the study. Chloroplasts in all of the cybrid clones were found to derive from the fertile parent. Mitochondrial DNA (mtDNA) segregation was complete in some but not all of the clones. In the cybrids, mtDNA was different from the parental plants. Physical mapping revealed recombination in a region which is not normally involved in the formation of subgenomic mtDNA circles. The role of treatments used to facilitate the recovery of cybrids, and of organelle compatibility in hybrid cytoplasm formation is discussed.read more
Citations
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Journal ArticleDOI
Inheritance and recombination of mitochondrial genomes in plants, fungi and animals.
TL;DR: It is argued that occasional biparental mitochondrial transmission may allow organisms to achieve the best of both worlds by facilitating mutational clearance but continuing to restrict the spread of selfish genetic elements.
Journal ArticleDOI
Evolution of plant mitochondrial genomes via substoichiometric intermediates.
TL;DR: In this article, a comparison of the modern fertile maize mitochondrial genome (N) with an ancestral maize mitochondrial genomes (RU) reveals a 12-kb duplication (containing the atpA gene) in the modern genome that is absent from the ancestor.
Journal Article
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoïd protein electrophoresis.
Journal ArticleDOI
An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum x L. pennellii F2 populations
J.P.W. Haanstra,C. Wye,H. Verbakel,Fien Meijer-Dekens,P. van den Berg,P. Odinot,A.W. van Heusden,Steve Tanksley,Pim Lindhout,J. Peleman +9 more
TL;DR: Two independent F2 populations of Lycopersicon esculentum×L.
Journal ArticleDOI
Intergeneric somatic hybridization and its application to crop genetic improvement
TL;DR: Somatic cell fusion has potential applications for crop genetic improvement by overcoming sexual incompatibility or reproductive barriers, and by realizing novel combinations of nuclear and/or cytoplasmic genomes.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Reversion of Male-Sterile T-Cytoplasm Maize to Male Fertility in Tissue Culture1
Journal ArticleDOI
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoid protein electrophoresis.
Journal ArticleDOI
Tripartite structure of the Brassica campestris mitochondrial genome
Jeffrey D. Palmer,C. R. Shields +1 more
TL;DR: Physical mapping studies indicate a novel sequence arrangement, consisting of three circular chromosomes, for the mitochondrial genome of Brassica campestris, and postulated to interconvert with the master chromosome via a co-integration-resolution pathway mediated by reciprocal recombination within the 2-kb repeat.
Journal ArticleDOI
Integration and organization of Ti plasmid sequences in crown gall tumors
TL;DR: The data suggest that the majority if not all of the T DNA is integrated into plant DNA, that preferred regions of the Ti plasmid serve as the points of attachment to plant DNA and that the TDNA can be linked to more than one site in the plant genome.
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